杜洛克猪氟烷基因PCR-RFLP分析  

PCR-RFLP of Halothane Gene in Duroc

作　　者：杨雪梅[1] 顾以韧[1] 陶璇[1] 梁艳[1] 吕学斌[1] 

机构地区：[1]四川省畜牧科学研究院,四川成都610066

出　　处：《养猪》2016年第4期82-84,共3页Swine Production

基　　金：四川省科技计划项目(2014NZ0092);四川省基本科研业务费专项资金(SASA2013B02);国家生猪产业技术体系-综合试验站(CARS-36);四川省财政运行专项(SASA2014CZYX001)

摘　　要：应用PCR方法,体外扩增了47头杜洛克猪的氟烷基因片段(659 bp),采用HhaⅠ内切酶对扩增产物进行了酶切多态性分析。结果表明,在试验猪群中都呈现氟烷基因阳性,氟烷显性基因频率Hal N频率和隐性基因Haln频率分别为1和0,该群体已实现氟烷隐性基因淘汰,可大面积供种。Using PCR method, the 47 Duroc pigs＇ halothane gene fragment（659 bp） had been amplified in vitro, which were carried through polymorphism analysis by Hha Ⅰ endonuclease. The results showed that in experimental pigs the halothane gene was positive, dominant gene frequency Hal N and recessive gene frequency were 1 and 0, the halothane recessive genes has been eliminated in group, large area for the species.

关 键 词：杜洛克猪 氟烷基因 PCR-RFLP 

分 类 号：S828[农业科学—畜牧学]