仙蓟化斑胶囊抗变态反应的作用机制及其对皮肤过敏反应、肥大细胞脱颗粒释放的抑制功效  

Mechanism of anti-allergy of Xianjihuaban capsule and its inhibitory effect on PCA and mast cells degranulation release

作　　者：李芊绵 任伟超[1] 刘志罡[1] 田明[1] 陈浩[1] 国紫薇 李芳[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国临床研究》2016年第8期1039-1041,1045,共4页Chinese Journal of Clinical Research

基　　金：黑龙江省科技计划项目(GC03C522)

摘　　要：目的针对仙蓟化斑胶囊抗变态反应的作用机制及其对皮肤过敏反应(PCA)、肥大细胞脱颗粒释放的抑制功效进行研究。方法选择130只大鼠作为研究的对象,取10只用于制备抗卵蛋白血清,其余120只随机均分为实验一组(抗卵蛋白血清皮内注射,PCA实验)、二组(抗卵蛋白血清皮下注射,颅骨骨膜肥大细胞脱颗粒实验)、三组(抗卵蛋白血清腹腔注射,肥大细胞释放组胺实验),各组均含对照组、高剂量观察组、低剂量观察组、阳性药物组四个亚组,每组10只大鼠。采用灌胃方式,对照组给予生理盐水,高、低剂量观察组分别给予仙蓟化斑胶囊1.45 g/kg、0.70 g/kg,阳性药物组给予息斯敏0.0009 g/kg。比较分析各实验中,大鼠的PCA、肥大细胞释放组胺、骨膜肥大细胞脱颗粒等指标在不同处理下(高、低剂量仙蓟化斑胶囊,息斯敏,生理盐水)的差异。结果在注射同一浓度血清时,高剂量、低剂量观察组和阳性药物组大鼠的皮斑直径均小于对照组大鼠(P均<0.05);但三个给药组之间皮斑直径无差异(P>0.05)。与对照组比较,高剂量、低剂量观察组和阳性药物组大鼠的骨膜肥大细胞脱颗粒比例均下降(P均<0.01);且按低剂量观察组、阳性药物组和高剂量观察组之序递降。与对照组比较,高剂量、低剂量观察组和阳性药物组大鼠的肥大细胞释放组胺OD值均下降(P均<0.01);且按低剂量观察组、阳性药物组和高剂量观察组之序递降。结论仙蓟化斑胶囊可缩小大鼠PCA的皮斑直径,抑制肥大细胞脱颗粒及组胺释放,具有抗变态反应的作用。Objective To research the mechanism of anti-allergy of Xianjihuaban capsule and its inhibitory effect on passive cutaneous anaphylaxis（ PCA） and mast cells degranulation release. Methods A total of 130 rats were selected as research objects. Ten rats in them were used to prepare the anti-ovalbumin serum,and the remaining 120 rats were randomly divided into three groups（ n = 40 each） ： experiment group A（ intradermal injection of anti ovalbumin serum for PCA test）,experiment group B（ subcutaneous injection of anti-ovalbumin serum for mast cells degranulation test of cranial periosteum） and experiment group C（ intraperitoneal injection of anti- ovalbumin serum for histamine release test of mast cells）,and each experiment group was re-divided into four sub-groups（ n = 10 each） ： control group,high-dose group,low-dose group and positive drug control group. By intragastric administration,normal saline was given in control group; 1. 45 g / kg and 0. 70 g / kg of Xianjihuaban capsule were given in high-dose group and low-dose group respectively; 0. 0009 g / kg of astemizol was given in positive drug control group. The indexes including PCA of rats,histamine release of mast cells,mast cells degranulation of cranial periosteum in different treatments（ high-dose and low-dose of Xianjihuaban capsule,astemizol and normal saline） were analyzed and compared. Results At injecting the same concentration of serum,the diameters of skin spots in high-dose group,low-dose group and positive drug control group were all significantly smaller than that in control group（ all P〈0. 05）,while there was no significant difference in three medication groups（ P〉0. 05）. Compared withcontrol group,the proportions of mast cells degranulation of cranial periosteum in high-dose group,low-dose group and positive drug control group decreased significantly（ all P〈0. 01） and went down progressively in the order of low-dose group,positive drug control group and high-dose group. Compared with control group

关 键 词：仙蓟化斑胶囊 抗变态反应 皮肤过敏反应 肥大细胞脱颗粒 组胺释放 

分 类 号：R285.5[医药卫生—中药学]