碳青霉烯类耐药肺炎克雷伯菌中KPC基因分布  

Prevalence of KPC gene in carbapenem-resistant Klebsiella pneumoniae isolates

作　　者：杨朵[1] 胡智颖[2] 辛续丽[2] 

机构地区：[1]首都医科大学附属北京世纪坛医院中心实验室,北京100038 [2]首都医科大学附属北京世纪坛医院检验科,北京100038

出　　处：《中国感染与化疗杂志》2016年第4期465-467,共3页Chinese Journal of Infection and Chemotherapy

摘　　要：目的检测碳青霉烯类耐药肺炎克雷伯菌中KPC基因分布情况,为此类菌感染的预防与控制提供理论依据。方法改良Hodge试验检测医院临床分离肺炎克雷伯菌中碳青霉烯酶表型;PCR法检测KPC基因的携带率。结果 642株碳青霉烯类抗生素耐药的肺炎克雷伯菌中,Hodge试验阳性率为96.6%。KPC基因的携带率为50.9%,均为KPC-2型。结论该医院临床分离的碳青霉烯类抗生素耐药肺炎克雷伯菌中KPC基因主要为KPC-2基因。Objective To investigate the prevalence of KPC gene in carbapenem-resistam K. pneumoniae isolates for better control of the relevant infections. Methods The carbapenemases were detected by Hodge test. KPC gene was tested by PCR. Results The results of Hodge test showed that 96.6% of the 642 strains of carbapenem-resistant K. pneumoniae could produce carbapenemases. And 50.9 % of the isolates were positive for KPC-2 gene. Conclusions KPC-2 gene is the primary KPC gene in carbapenem- resistant K. pneumoniae in this hospital.

关 键 词：肺炎克雷伯菌 碳青霉烯酶 KPC基因 Hodge试验 

分 类 号：R440[医药卫生—诊断学]