抗细胞膜DNA抗体与其他自身抗体的检测及相关分析  

Detection and Correlation Analysis between Anti-cell Membrane DNA Antibody and Other Autoantibodies

作　　者：胡静[1] 李立新[1] 武永康[1] 魏大鹏[2] 彭晓东[1] 

机构地区：[1]四川大学华西医院实验医学科,成都610041 [2]四川大学华西基础与法医学院免疫教研室,成都610041

出　　处：《华西医学》2016年第8期1372-1375,共4页West China Medical Journal

基　　金：四川省科技厅科研课题(2016JY0035)~~

摘　　要：目的分析抗细胞膜DNA(m DNA)抗体与其他自身抗体的相关性,评价其对系统性红斑狼疮(SLE)的诊断意义。方法收集2015年1月-8月就诊的254例自身免疫性疾病患者血清,包括SLE 106例,类风湿关节炎(RA)80例,混合性结缔组织病(MCTD)32例,干燥综合征(SS)29例,多发性肌炎/皮肌炎(PM/DM)7例;另再收集20例正常对照者血清。分别用间接荧光免疫法检测抗m DNA抗体、抗双链DNA(ds DNA)抗体、抗核抗体(ANA)及抗角蛋白抗体(AKA),酶联免疫吸附测定法检测抗环瓜氨酸肽(CCP)抗体,速率散射比浊法检测类风湿因子(RF),免疫印迹法检测抗Sm抗体。结果抗m DNA抗体在SLE中的阳性率为72.6%(77/106),显著高于RA[5.0%(4/80)]、MCTD[18.7%(6/32)]、SS[14.7%(4/29)]、PM/DM[0.0%(0/7)]以及健康对照组[0.0%(0/20)],差异有统计学意义(P<0.001);抗m DNA抗体对SLE诊断的灵敏度、特异度和诊断效率分别为72.6%、91.7%和84.3%;抗m DNA抗体与ANA、抗ds DNA抗体及抗Sm抗体显著相关(P<0.001),与抗CCP抗体、AKA及RF无相关性(P>0.05)。结论抗m DNA抗体与SLE相关自身抗体密切相关,对SLE诊断具有高度灵敏度和特异度。Objective To analyze the correlation between anti-cell membrane DNA （mDNA） antibodies and other autoantibodies and estimate its diagnosing significance for systemic lupus erythematosus （SLE）. Methods From January to August 2015, the sera samples from 254 patients with various autoimmune diseases, including 106 SLE, 80 rheumatoid arthritis （RA）, 32 mixed connective tissue disease （MCTD）, 29 Sjogren＇s syndrome （SS）, 7 polymyositis/ dermatomyositis （PM/DM） and 20 healthy controls, were collected. The anti-mDNA antibody, anti-dsDNA antibody, antinuclear antibody （ANA） and anti-keratin antibody （AKA） were detected by indirect immunofluorescent assay; anti- cyclic citrylinated peptide antibody （CCP） antibody was detected by enzyme-linked immuno sorbent assay; rheumatoid factor （RF） was detected by rat scatter turbidimetry assay; and anti-Sm antibody was detected by Western blotting method. Results Anti-mDNA antibody was found in 77 of 106 SLE （72.6%）, 4 of 80 RA （5.0%）, 6 of 32 MCTD （18.7%）, 4 of 29 SS （14.7%）, 0 of 7 PM/DM （0.0%） and 0 of 20 healthy controls （0.0%）, respectively. It＇s notable higher in SLE than that in the others （P 〈 0.001）. The sensitivity, specificity and diagnosis efficiency of anti-mDNA antibody for SLE were 72.6%, 91.7% and 84.3%, respectively. Anti-mDNA antibody was significantly correlated with ANA, anti-dsDNA antibody and anti-Sm antibody （P 〈 0.001）, while it had no significant correlation with anti-CCP antibody, AKA and RF （P 〉 0.05）. Conclusion Anti-mDNA antibody is closely related with other SLE associated antibodies and with high sensitivity and specificity for SLE diagnosis.

关 键 词：抗细胞膜DNA抗体 系统性红斑狼疮 相关性 灵敏度 特异度 

分 类 号：R593.241[医药卫生—内科学]