金丝桃素在大鼠体内的代谢产物  

Metabolites of hypericin in rats in vivo

作　　者：赵娟芝[1,2] 王聪[1,2] 李进典[1,2] 张东建[2] 张健[2] 殷志琦[1] 

机构地区：[1]中国药科大学天然药物化学教研室&天然药物活性组分与药效国家重点实验,南京210009 [2]江苏省中医药研究院转化医学实验室,南京210028

出　　处：《中国药科大学学报》2016年第4期457-462,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.81473120);"华海药业研究生创新基金"资助项目~~

摘　　要：分离鉴定金丝桃素在大鼠胆汁中的代谢产物以及在坏死肝脏组织中的存在形式。正常大鼠和肝脏缺血再灌注损伤模型大鼠经静脉注射10 mg/kg金丝桃素后,收集正常大鼠0～12 h胆汁以及坏死模型大鼠24 h坏死肝脏组织,对生物样品预处理后,采用高效液相色谱-高分辨飞行时间质谱（HPLC-TOF/MS）联用技术进行分析。在大鼠胆汁中鉴定出金丝桃素原形（M0）和3个葡萄糖化代谢产物（M1、M2、M3）,而在坏死肝脏组织中仅鉴定出金丝桃素原形。结果表明,金丝桃素在大鼠胆汁中主要以原形和葡萄糖化形式存在,在坏死组织中主要以原形存在。The study aimed to separate and identify the metabolites of hypericin in the bile and necrotic tissues in rats. After intravenous injection of 10 mg/kg hypericin, 0-12 h bile of normal rats and 24 h necrotic liver of rats with reperfused hepatic infarction were collected, and metabolites of rats were analyzed by high performance liquid chromatography coupled with electrospray tandemtime of dight mass spectrometry（ HPLC-TOF/MS）. The prototype（M0） and three glycosylation metabolites（ M1, M2, M3） of hypericin in rat bile and the parent compound in rat necrotic liver were detected and identified. Results indicated that prototype and glycosylation of hypericin were the major metabolic form in rat bile and the parent compound was found only in necrotic tissues. Key words hypericin; metabo.lites; HPLC-TOF/MS; glycosylation; isolation and identification

关 键 词：金丝桃素 代谢产物 高效液相色谱-飞行时间质谱 葡萄糖化 分离鉴定 

分 类 号：R285.5[医药卫生—中药学]