海南高良姜居群遗传多样性和亲缘关系分析  

Genetic diversity and relationship of Alpinia officinarum hance populations

作　　者：潘坤[1] 高炳淼[1] 王阿超 关薇薇[1,2] 

机构地区：[1]海南医学院,海南海口571199 [2]海南省热带药用植物研究开发重点实验室,海南海口571199

出　　处：《生物技术》2016年第4期380-385,共6页Biotechnology

基　　金：海南省自然科学基金项目("海南岛菠萝蜜种质遗传多样性与指纹图谱构建";No.314162);海南医学院培育基金项目("海南高良姜居群多样性分析和指纹图谱构建";No.HY2014-022);海南医学院引进人才科研启动经费

摘　　要：[目的]对海南的8个高良姜居群进行遗传多样性和亲缘关系分析。[方法]利用ISSR分子标记对其进行PCR扩增,采用NTSYS和POPGENE等软件进行数据分析。[结果]高良姜居群水平的多态位点百分率为31.45%,物种水平为65.61%;居群遗传分化系数为0.338 9,聚类树种遗传相似度为0.903处,除海口的两个居群单独聚为一类,其余的6个居群聚类方式没有地理相关性。[结论]海南高良姜较低的(65.61%)和居群外变异(33.89%)等数据揭示了其遗传多样性不高,但发生了一定水平的居群内遗传变异。[ Objectivel Genetic diversity and relationship of 8 populations of Alpinia officinarum from Hainan was analyzed in this study. [ Methods ] ISSR （inter simple sequence repeats） technology was used in PCR, the software NTSYS and POPGENE was used in data analysis. [ Results ] The percentage of polymorphic loci was calculated, within populations was 31.45% , and within species was 65.61%. The coefficient of gene differentiation was 0. 338 9. All of the populations were found grouped into two clusters at the genetic similarity as 0. 903, one of them consists two populations from Haikou, and the other consists the rest populations that shown no geographical relation. [ Conclusion] With the lower percentage of polymorphic loci （65.61% ） with- in species and variation among populations （33.89%） ,which supports the conclusion that genetic diversity of Alpinia officina- rum from Hainan was moderate, but some genetic variations were found within population.

关 键 词：高良姜 居群 ISSR 遗传多样性 亲缘关系 

分 类 号：Q349[生物学—遗传学]