简单高效获取人脐静脉内皮细胞的方法研究  

A simple and efficient method for harvesting human umbilical vein endothelial cells

作　　者：霍永宝 叶子冠[1] 陈瑞林[1] 陶怡[1] 

机构地区：[1]广州医科大学附属第二医院风湿免疫科,广东广州510260

出　　处：《广州医科大学学报》2016年第3期37-39,共3页Academic Journal of Guangzhou Medical University

基　　金：2013年广州医科大学博士启动项目(20BC51);2015年广东省自然科学基金(2015A030313459)

摘　　要：目的:探索简单高效的获取人脐静脉内皮细胞(HUVECs)的方法。方法:采用Ⅱ型胶原酶对健康产妇正常分娩的脐带进行消化,分离出HUVECs,予含10%胎牛血清、1%内皮细胞生长液的PRM 1640培养液,在37℃、5% CO_2条件下培养,分别进行细胞鉴定及活力检测。结果:分离获取的HUVECs呈典型的"鹅卵石、铺路石"样,有特异性vWF抗原表达,细胞活力好。结论:Ⅱ型胶原酶对健康产妇正常分娩的脐带进行消化分离,可获取大量纯度高、细胞活力好的HUVECs,简单高效、可行性强。Objective：To explore for a simple and efficient method for harvesting human umbilical vein endothelial cells （ HUVECs） . Methods：HUVECs were isolated by Type Ⅱ collagenase digestion of umbilical cord from healthy women who underwent normal delivery, and then were cultured in PRM 1640 medium liquid containing 10% fetal calf serum and 1% endothelial cell growth liquid, at 37 degrees Celsius under 5% CO2 condition. The cultured cells were identified and assayed for viability. Results： After culture, the isolated HUVECs showed a typical pattern of cobblestones or paving stones, specifically expressed vWF antigens, and were with good cell viability. Conclusion： Type Ⅱ collagenase digestion of umbilical cord from healthy women who underwent normal delivery may be a simple, efficient and feasible method which massively yields highly-purified and viable HUVECs.

关 键 词：人脐静脉内皮细胞 细胞培养 原代 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]