农杆菌介导禾本科植物遗传转化的研究进展  

Progress of Agrobacterium-mediated Genetic Transformation in Gramineae

作　　者：张瑜[1] 鄢家俊[1] 白史且[1] 张建波[1] 张劲[1] 彭燕[2] 李达旭[1] 

机构地区：[1]四川省草原科学研究院,四川成都611731 [2]四川农业大学草业科学系,四川雅安625014

出　　处：《草业与畜牧》2016年第4期4-9,共6页Pruataculture & Animal Husbandry

基　　金：斑茅关键木质素合成酶基因克隆;RNA干扰及抗逆性表达研究(31302026);四川省公益性科研院所基本科研业务费项目:新型能源草木质素代谢相关基因克隆及其功能研究共同资助

摘　　要：农杆菌介导的遗传转化是目前禾本科植物进行转基因的有效手段,高效遗传转化体系建立的关键是优化T-DNA导入的各因素。文中主要从农杆菌菌株及载体、植物受体的选择、侵染与共培养、抗生素及添加剂的筛选等方面详细阐述了农杆菌介导植物遗传转化的主要影响因素,为实践操作提供了理论依据。Currently agrobacterium-mediated gene transferring is one of efficient methods in transgenie gramineous plants. The key to establish an agrobacterium-mediated high efficient genetic transformation system is to optimize the factors of transforming T-DNA into plant, including the factors of agrobacterium strains and vectors, selection of plant receptors, infection and co-culture, and screening of antibiotics and additives. This paper stated the role factors in agrobacterium-mediated plant genetic transformation in details, in order to provide theoretical basis for the practical operation.

关 键 词：农杆菌 遗传转化 禾本科 影响因素 

分 类 号：Q785[生物学—分子生物学]