西藏扁芒菊总多糖的理化性质及免疫增强活性  

Physicochemical Property and Immuno-enhancing Activity of Polysaccharides from Waldheimia glabra( Decne. ) Regel

作　　者：高慧琴[1,2] 德吉[3] 凌丽君[2] 张庆琳[2] 卢燕[2] 陈道峰[2] 

机构地区：[1]山西医科大学药学院,太原030001 [2]复旦大学药学院,上海201203 [3]西藏大学理学院,拉萨850000

出　　处：《天然产物研究与开发》2016年第8期1309-1312,共4页Natural Product Research and Development

基　　金：西藏自治区科技计划"自然科学基金"(Z2012Z01G03);科技部十二五"重大新药创制"重大科技专项(2012ZX09301001-003)

摘　　要：用水提醇沉法提取了西藏扁芒菊总多糖,并利用一系列物理化学方法对总多糖的组成进行表征,探究其对免疫功能的影响。结果表明:西藏扁芒菊总多糖由半乳糖、阿拉伯糖、葡萄糖、甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、核糖组成,多糖分子量为1110000。其多糖含量、糖醛酸含量、蛋白含量分别为63.6%、20.76%、11.24%。进一步研究表明西藏扁芒菊总多糖能够显著增强巨噬细胞NO分泌功能,具有免疫增强活性,为西藏扁芒菊多糖的开发利用提供了依据。The crude polysaecharides of Waldheimia glabra （Deene.） Regel （W. glabra） were extracted by water ex- traction and ethanol precipitation method and characterized with a series of physical and chemical methods. The effect of the polysaccharides on the immunologic functions was then investigated. The results showed that the polysaceharides were composed of galactose, arabinse, glucose, mannose, rhamose, glucuronic acid, galacturonic acid and ribosc and the rela- tive molecular mass was about 1110000. The contents of polysaccharides, uronic acid and protein of the crude polysae- charides were 63.60% ,20.76% and 11.24%, respectively. The in-depth study indicated that the polysaccharides obvi- onsly promoted the NO secretion of macrophage and thus enhanced immune activity. This study provided the basis for the development and utilization of polysaccharides from W. glabra.

关 键 词：西藏扁芒菊 总多糖 理化性质 免疫增强 

分 类 号：S859.79[农业科学—临床兽医学]