免疫印迹法在老年人梅毒诊断中的应用  

Application of Western Blot in the Diagnosis of Syphilis in the Elderly

作　　者：王文雯[1] 周雅[1] 

机构地区：[1]贵州省人民医院皮肤科,贵州贵阳550002

出　　处：《中国皮肤性病学杂志》2016年第9期922-923,926,共3页The Chinese Journal of Dermatovenereology

基　　金：贵州省科技厅科技基金(黔科合SY字[2008]3029)

摘　　要：目的采用免疫印迹法(WB)对临床梅毒血清学检测TPPA阳性且RPR阴性的老年人进行复测,探讨该人群是否存在梅毒感染。方法采集2012年11月-2014年10月就诊于贵州省人民医院患者,对84例RPR(-)、TPPA(+)的老年人血清标本运用免疫印迹法(WB)(TP-IgG)进行复测,对实验结果进行统计学分析。结果 WB法(TP-IgG)复测有79例阳性,5例可疑阳性,阳性率94.05%,与TPPA法符合率94.05%。WB(TP-IgG)法四种特异性蛋白均有表达。在TPPA滴度不同的情况下,TP17和TP15表达较高,TP47表达较其他3种蛋白低。结论在对TPPA阳性且RPR阴性的老年人群梅毒检测结果分析中,TPPA法与WB法检测有较高符合率。对用WB(TP-IgG)复测结果为可疑阳性的标本,在临床上需长期随访,必要时建议用TP-IgM检测,为是否进行驱梅治疗提供依据。Objective Using Western blot （WB） to detect the syphilis infection in the elderly with RPR negative and TPPA positive. Methods Western blot was used to detect TP-IgG in 84 elderly with RPR negative and TPPA positive, tested at Guizhou provincial people＇ s hospital November 2012 to October 2014. Results WB showed 79 positive （94. 05% ） and 5 suspected positive. WB detected all four specific proteins in these elderly. Under different titer conditions, the expression levels of TP17 and TP15 were higher while TP47 expression was lower among the four proteins. Conclusion In the diagnosis of syphilis with RPR negative and TPPA positive in the elderly, TPPA method has high coincidence rate with WB method. The patients with suspected TP-IgG positive by WB should be followed up in clinic. If necessary, TP-IgM test should be performed to determine whether antisyphilitic treatment is required.

关 键 词：老年人 梅毒血清学检查 梅毒螺旋体颗粒凝集试验 免疫印迹法 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]