槲寄生内生菌IA-4-2及其代谢产物的分离、鉴定和神经氨酸酶抑制作用考察  

Isolation,Identification and Neuraminidase Inhibition Test of Secondary Metabolite Produced by Mistletoe Endophyte IA-4-2

作　　者：杨巧利[1] 郭菁菁[1,2] 代斌玲 俞杭苏 钱朝东[1] 

机构地区：[1]浙江中医药大学,杭州310053 [2]绍兴市中心医院

出　　处：《中国药师》2016年第8期1442-1445,共4页China Pharmacist

基　　金：浙江中医药大学大学生创新创业训练计划项目(编号:781110V005)

摘　　要：目的:研究对神经氨酸酶具有抑制作用的槲寄生内生菌次生代谢产物。方法:采用AB-8大孔吸附树脂、C_(18)-SPE柱层析结合制备型HPLC等手段进行分离纯化,根据GC-MS和HPLC等分析手段确定化合物结构,并且利用神经氨酸酶抑制药筛选试剂盒测定活性。结果:从槲寄生内生菌IA-4-2中获得对神经氨酸酶具有较强抑制作用的化合物,经结构鉴定为吲哚-3-甲醛,其对神经氨酸酶的IC50为128.2μg獉ml^(-1)。结论:槲寄生内生菌次生代谢产物吲哚-3-甲醛对神经氨酸酶抑制作用明显,值得进一步研究。Objective： To study the secondary metabolite of mistletoe endophyte with neuraminidase inhibition. Methods： The secondary metabolite of mistletoe endophyte was isolated and purified by AB-8 macroporous absorption resin,C_（18）-SPE column chromatography and preparative HPLC. The structure was elucidated on the basis of GC-MS and HPLC analysis,and the inhibitory activity against neuraminidase was determined by neuraminidase inhibitor screening kit. Results： One secondary metabolite with the inhibitory activity against neuraminidase was found in the culture of mistletoe endophyte IA-4-2,and its structure was determined as indole-3-carboxaldehyde. The IC50 for neuraminidase was 128. 2 μg · ml^（-1）. Conclusion： Indole-3-carboxaldehyde produced by mistletoe endophyte shows potential inhibitory activity against neuraminidase,which is worthy of further study.

关 键 词：槲寄生内生菌 次生代谢产物 神经氨酸酶抑制药 吲哚-3-甲醛 

分 类 号：R282.71[医药卫生—中药学]