抗毒合剂HPLC指纹图谱的研究  

Fingerprint of Kangdu Mixture by HPLC

作　　者：杜向红[1] 杜云锋[1] 张伟[1] 李国峰[1] 刘伟[2] 陈宁[2] 

机构地区：[1]河南省人民医院,郑州450008 [2]河南中医学院分析测试中心

出　　处：《中国药师》2016年第8期1489-1493,共5页China Pharmacist

基　　金：河南省科技厅重点科技攻关项目(编号:122102310069)

摘　　要：目的:建立了抗毒合剂的指纹图谱分析方法。方法:采用高效液相色谱法,色谱条件:Agilent C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相:0.1%磷酸水-乙腈,梯度洗脱,流速:1.0 ml·min^(-1),柱温:25℃,检测波长:290 nm。结果:在选定的色谱条件下确定了17个共有峰构成10批抗毒合剂的指纹特征指纹图谱,各批样品的相似度均大于0.9。结论:该法精密度高,重复性好,可为抗毒合剂的指纹图谱评价提供依据。Objective： To establish a fingerprint analysis method for Kangdu mixture. Methods： An HPLC method was used with the following chromatographic conditions： an Agilent C_（18） chromatographic column（ 150 mm × 4. 6 mm,5 μm） was applied,the mobile phase was 0. 1% phosphoric acid water and acetonitrile with gradient elution at a flow rate of 1. 0 ml·min^（-1）,the column temperature was 25 ℃,and the detection wavelength was 290 nm. Results： Under the selected chromatographic conditions,17 common peaks were identified to construct the fingerprint for 10 batches of Kangdu mixture,and the similarity of the samples was above 0. 9. Conclusion：The established method is with high precision and good reproducibility,which can be applied in the fingerprint evaluation of Kangdu mixture.

关 键 词：抗毒合剂 高效液相色谱法 指纹图谱 

分 类 号：TQ460.72[医药卫生—药物分析学]