HPLC-ELSD法测定赤雹不同药用部位中山柰酚-3-O-β-D-吡喃葡萄糖-7-O-α-L-鼠李糖苷的含量  

Determination of Kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in Different Medicinal Parts of Thladiantha Dubia Bunge by HPLC-ELSD

作　　者：程东岩[1] 陈昕[2] 王隶书[1,2] 高军[1] 

机构地区：[1]吉林省中医药科学院,长春130012 [2]长春中医药大学

出　　处：《中国药师》2016年第8期1592-1593,1599,共3页China Pharmacist

基　　金：吉林省科技厅自然科学基金项目(编号:20140101127JC)

摘　　要：目的:建立赤雹不同药用部位(根、茎、果实)中山柰酚-3-O-β-D-吡喃葡萄糖-7-O-α-L-鼠李糖苷含量的PLC-ELSD测定方法。方法:采用高效液相色谱法-蒸发光散射检测器检测。色谱柱:Alltima C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(19∶81);柱温:30℃,流速:0.8 ml·min^(-1)。ELSD条件:漂移管温度:105℃;载气流速:2.8 L·min^(-1)。结果:山柰酚-3-O-β-D-吡喃葡萄糖-7-O-α-L-鼠李糖苷在51.15~920.70μg·ml^(-1)浓度范围内线性关系良好(r=0.999 8);平均回收率根、茎、果实分别为100.1%,100.5%,101.1%,RSD分别为1.82%,1.79%,1.54%(n=6)。结论:该方法简便、准确,可用于赤雹药材的质量控制。Objective： To establish the determination method for kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside in the different medicinal parts（ root,stem and fruit） of Thladiantha dubia Bunge by HPLC-ELSD. Methods： The HPLC-ELSD method was set up by using an Alltima C18column（ 250 mm × 4. 6 mm,5 μm）,the mobile phase was acetonitrile-water（ 19 ∶ 81） with a flow rate of 0. 8 ml·min^（-1）,and the column temperature was 30℃. The temperature of drift tube heater in ELSD was 105 ℃ and the flowing rate of gas was 2. 8 L·min^（-1）. Results： The linear range of kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside was 51. 15-920. 70 μg·ml^（-1）（ r = 0. 999 8）. The average recovery of root,stem and fruit was 100. 1%,100. 5% and 101. 1% with RSD of 1. 82%,1. 79% and 1. 54%（ n = 6）,respectively. Conclusion： The method is simple and accurate,which can be used for the quality control of Thladiantha dubia Bunge.

关 键 词：赤雹 药用部位 山柰酚-3-O-β-D-吡喃葡萄糖-7-O-α-L-鼠李糖苷 高效液相色谱法-蒸发光散射检测法 

分 类 号：R927.2[医药卫生—药学]