Metatranscriptome of the protistan community in Reticufitermes flaviceps  

作　　者：Xiao-Jing Liu Min Che Lei Xie Shuai Zhan Zhi-Hua Zhou Yong-Ping Huang Qian Wang 

机构地区：[1]Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai [2]College of Science, Air Force Engineering University, Xi'an [3]Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China

出　　处：《Insect Science》2016年第4期543-547,共5页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （Grants 31472046, 31172153）.

摘　　要：The hindgut of lower termites harbors various symbiotic protists, which per- form varied functions in lignocellulose decomposition. As termites are social insects, the species and numbers of these flagellated protists in the termite gut vary among the different castes. Juvenile hormones （JHs） can regulate caste differentiation in termites. In this study, we used the juvenile hormone analog fenoxycarh to induce termite workers （Reticulitermesflaviceps） to differentiate into pre-soldiers. A metatranscriptomic investigation of the protistan community was then performed by 454 pyrosequencing. From a thorough analysis based on 597 312 generated reads, we found that the starch and sucrose metabolism pathway was the most abundant pathway across the metatranscriptome. The current study demonstrates that the metatranscriptome of the protistan community in termites contains an abundance of lignocellulase, which plays a vital role in termite nutrition.The hindgut of lower termites harbors various symbiotic protists, which per- form varied functions in lignocellulose decomposition. As termites are social insects, the species and numbers of these flagellated protists in the termite gut vary among the different castes. Juvenile hormones （JHs） can regulate caste differentiation in termites. In this study, we used the juvenile hormone analog fenoxycarh to induce termite workers （Reticulitermesflaviceps） to differentiate into pre-soldiers. A metatranscriptomic investigation of the protistan community was then performed by 454 pyrosequencing. From a thorough analysis based on 597 312 generated reads, we found that the starch and sucrose metabolism pathway was the most abundant pathway across the metatranscriptome. The current study demonstrates that the metatranscriptome of the protistan community in termites contains an abundance of lignocellulase, which plays a vital role in termite nutrition.

关 键 词：juvenile hormone analogues metatranscriptome PROTEST Reticulitermes flaviceps lignocellulase 

分 类 号：Q145[生物学—生态学] Q969.296[生物学—普通生物学]