玉米变性淀粉两种不同DNA提取方法的比较研究  

Comparison of two different extraction methods of genomic DNA from modified maize starch

作　　者：张娟[1] 杨纯佳 周臣清 綦艳[1] 

机构地区：[1]广东产品质量监督检验研究院国家食品质量监督检验中心(广东),广东佛山528300

出　　处：《中国酿造》2016年第8期53-56,共4页China Brewing

基　　金：中国南方智谷引进创新团队项目(201209)

摘　　要：采用两种不同方法对玉米变性淀粉基因组DNA提取比较,并经特异性引物进行实时荧光定量-聚合酶联反应(qRT-PCR)检测,从中选择出更适合玉米变性淀粉后续转基因成分检测的DNA提取方法。通过采用磁珠法和试剂盒法提取玉米变性淀粉基因组DNA,比较不同方法的提取效果。结果表明,试剂盒法的提取效果优于磁珠法。实时荧光定量PCR结果显示,试剂盒法提取的基因组DNA均可扩增出标准的S形曲线,而磁珠法提取的基因组DNA则无任何扩增信号。该研究为检测玉米变性淀粉中的转基因成分奠定了基础。The different methods of genome DNA from modified maize starch were compared. The specific primers were detected by quantitative re- al-time polymerase chain reaction （qRT-PCR）, and the DNA extraction method which was more suitable for subsequent gmo detection modified maize starch was determined. The genome DNA from modified maize starch was extracted by paramagnetic particle and kit method, and the extrac- tion effects of different methods were compared. The results showed that the genome DNA extracted by kit method could be amply standard S-shaped curve, but the genome DNA extracted by magnetic beads method could not find any amplification signal. This study established the founda- tion for detection of genetically modified components from modified maize starch.

关 键 词：玉米变性淀粉 DNA提取 转基因成分 检测 

分 类 号：Q781[生物学—分子生物学]