亚慢性铝暴露对大鼠学习记忆及大脑皮质c-fos mRNA和c-Fos蛋白表达的影响  

Effects of subchronic aluminum exposure on learning and memory c-fos mRNA and c-Fos protein expression of cerebral cortex in rats

作　　者：张立丰[1] 赵丽妮[2] 白春玉[1] 张迪[1] 

机构地区：[1]沈阳医学院公共卫生学院儿少卫生与妇幼保健学教研室,沈阳110034 [2]沈阳医学院基础医学院药理学教研室,沈阳110034

出　　处：《神经解剖学杂志》2016年第4期447-451,共5页Chinese Journal of Neuroanatomy

基　　金：辽宁省教育厅科学技术研究项目(L2015544);沈阳医学院科技基金项目(20153043)

摘　　要：目的:探讨亚慢性铝暴露对大鼠学习记忆能力的影响,分析大脑皮质即刻早基因表达变化,初步探索铝对认知功能损害的毒性作用及机制。方法:随机将孕大鼠分为对照组和低、中、高染铝组,染毒组仔鼠出生后首日连续染毒3个月,然后各组大鼠进行水迷宫测试,铝含量检测,皮质神经细胞病理学观察,皮质c-fos mRNA和蛋白表达水平检测。结果:铝含量随染毒剂量增加而升高;染铝组学习记忆能力明显低于对照组,并呈剂量-效应关系;染铝组皮质神经细胞呈现神经纤维稀疏、神经元细胞脱失甚至空泡样变性等病理改变;中、高剂量组皮质区c-fos mRNA表达水平分别低于对照组和低剂量组。染铝组皮质区c-Fos蛋白表达水平低于对照组。随染毒剂量增加,c-fos mRNA和蛋白表达水平降低并具有一定的相关性。结论:亚慢性铝暴露损害大鼠学习记忆能力,其机制可能与铝造成皮质神经细胞功能减弱(即刻早基因c-fos mRNA和蛋白表达水平下降)有关。Objective： To investigate the effects of subchronic exposure to aluminium（ Al） on learning and memory,and preliminarily explore the corresponding toxic mechanism underlying cognitive impairments in rats by evaluation of immediate early gene expression in cerebral cortex. Methods： Pregnant female rats were divided randomly into four groups：control,low-medium- and high-dose Al groups. Pups of Al groups were administrated with AlC l3 for 3 months starting from the postnatal day 1. Then,test of Morris water maze and assay of Al concentrations were performed,and cortical neuron morphology,c-fos gene and c-Fos protein expressions were examined. Results： The Al concentrations increased with the increasing Al dose. Learning and memory of pups in Al groups were significantly lower than those in control group,showing a dose-effect relationship. The neuronal morphology in cerebral cortex exhibited pathological changes such as decreased density of nerve fibers,depigmentation and even vacuolar degeneration after Al treatment. c-fos mRNA expression of cerebral cortex in rats of medium- and high-dose Al groups was significantly lower than that in control- and low-dose groups,respectively. c-Fos protein expression of cerebral cortex in Al exposed groups rats was significantly lower as compared with that in control group. c-fos mRNA and protein expression of cerebral cortex decreased with increasing Al dose,demonstrating a dose-effect relationship manner to some degree. Conclusion： Subchronic Al exposure could injurelearning and memory of rats,mechanically related to decreased level of neuronal function,as was presented by the Al-induced decrease in c-fos mRNA and protein expression.

关 键 词：铝 皮质 学习记忆 C-FOS 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]