SAHA和TRAIL联合应用对雌激素受体阳性乳腺癌细胞MCF-7生长抑制的分子机制  

Effects of Combination with SAHA and TRAIL Treatment on Cell Growth of ER Positive Breast Cancer Cell Line MCF-7

作　　者：冯秀艳[1,2] 晏林[3] 周伟强 

机构地区：[1]辽宁省环境污染与微生态重点实验室,沈阳110034 [2]沈阳医学院附属第二医院内分泌科,沈阳110002 [3]沈阳医学院预防医学院,沈阳110034

出　　处：《中国药学杂志》2016年第16期1373-1378,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81172509);沈阳医学院科技基金项目(20121003)

摘　　要：目的探讨SAHA(suberoylanilide hydroxamic acid)和肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)联合作用抑制雌激素受体阳性乳腺癌细胞MCF-7生长的分子机制。方法以乳腺癌细胞株MCF-7为研究对象,应用自动细胞分析仪测定SAHA和TRAIL联合作用对乳腺癌细胞活力和细胞凋亡的影响并对细胞周期进行分析;应用实时定量PCR及固相凋亡抗体芯片检测MCF-7细胞凋亡蛋白表达。结果 SAHA和TRAIL联合作用可显著降低MCF-7细胞活力,抑制细胞增殖,并诱导细胞凋亡的产生。SAHA和TRAIL对MCF-7细胞周期的影响主要集中在G_0/G_1期。结论 SAHA和TRAIL联合作用对乳腺癌细胞生长有抑制作用。OBJECTIVE To study the effects of suberoylanilide hydroxamic acid （SAHA） and TRAIL treatment on cell proliferation and apoptosis for ER positive breast cancer cell line MCF-7, METHODS Human breast cell lines （MCF-7） were evaluated for the expressions of cell viability, cell apoptosis and cell cycle by muse cell analyzer. The mRNA levels of related apoptotic factors in MCF-7 cells were detected by real time PCR and solid phase apoptosis antibody microarray. RESULTS After the combination with SAHA and TRAIL, the ability of cell proliferation and cell viability were depressed, and the cell apoptosis was induced. The cell cycle assay showed that the MCF-7 cells were arrested in G0/G1 phase with SAHA and TRAIL treatment. CONCLUSION The combinatorial treatment of SAHA and TRAIL has a significantly inhibitory effect on cell growths of ER positive breast cancer MCF-7 cell.

关 键 词：雌激素受体阳性乳腺癌 细胞凋亡 SAHA 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R965[医药卫生—药理学]