燃煤污染型地方性氟中毒患者血细胞hMLH1与MGMT mRNA表达改变  

The effect of endemic fluorosis caused by coal burning on the expression of hMLH1 and MGMT mRNA

作　　者：王煜蘅[1] 张婷[1] 吴昌学[1] 李毅[1] 官志忠[1,2] 齐晓岚[1] 

机构地区：[1]贵州医科大学分子生物学重点实验室,贵阳550004 [2]贵州医科大学病理学教研室,贵阳550004

出　　处：《重庆医学》2016年第24期3348-3350,共3页Chongqing medicine

基　　金：国家科技支撑计划项目(2013BAI05B03);国家自然科学基金资助项目(81160335);贵州省科技厅重点项目[黔科合计Z字(2012)4010];贵州省科技计划课题(国际合作项目)资助[黔科合外G字(2011)7014号]

摘　　要：目的探讨燃煤污染型地方性氟中毒(简称燃煤型地氟病)对血液中错配修复基因(hMLH1)及O(6)-甲基鸟嘌呤DNA甲基转移酶基因(MGMT)mRNA表达水平的影响。方法在贵州省毕节地区燃煤型地氟病区抽取45例患者,按其氟斑牙和氟骨症严重程度分为轻、中、重度组各15例。在毕节地区非病区抽取15名村民作为对照组。实时荧光定量PCR技术检测各组血样本MGMT、hMLH1mRNA水平。结果燃煤型氟中毒轻、中、重度3个组中hMLH1及MGMT mRNA水平与对照组相比明显降低,差异有统计学意义(P<0.05),且随着氟中毒程度的加重,其降低程度更加明显。组间两两比较,随着中毒程度加重,表达水平明显降低(P<0.05)。结论 MGMT与hMLH1mRNA水平在燃煤型氟中毒所造成的DNA损伤及修复机制中可能有负面作用。Objective To discuss the impact of endemic fluorosis caused by coal burning on the DNA repair gene human mis- match repair gene（hMLH1） and O（6）-methylguanine-DNA methyltransferase（MGMT） mRNA level in blood. Methods Forty-five villagers selected from Bijie area of Guizhou Province were divided into three groups as the low-dose fluoride group,the middle-dose fluoride group, the high-dose fluoride group based on the severity of dental and skeletal fluorosis,and each group with 15 cases. Fif- teen villagers from the non-fluorosis area of Bijie area were seiected as control group. The MGMT and hMLH1 mRNA were quanti- rated with the real-time quantitative PCR technology. Results The mRNA expression level of MGMT,MLH1 mRNA from the pa- tients in fluorosis region decreased compared to control group（P〈0. 05） ,and had a positive correlation with the severity of fluoro- sis,which was significantly different in group comparison（P〈0.05）： Conclusion The reduction of MGMT and hMLH1 mRNA may play an important role in the mechanism of DNA injury and repair,which may be related to DNA damage arose from fluoride caused by coal burning.

关 键 词：O(6)-甲基鸟嘌呤DNA甲基转移酶 错配修复基因 氟中毒 

分 类 号：R599[医药卫生—内科学]