余甘子醇提物抑制人胃癌株AGS细胞增殖和诱导细胞凋亡的作用  

Effects of Ethanol Extract of Phyllanthi Fructus on Proliferation and Apoptosis of Human Gastric Cancer AGS Cells

作　　者：罗兰[1] 林久茂[2] 魏丽慧[2] 李琼瑜[2] 薛曼华[1] 彭军[2] 

机构地区：[1]福建卫生职业技术学院药学系,福州350101 [2]福建中医药大学中西医结合研究院,福州350122

出　　处：《中国现代应用药学》2016年第8期989-993,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：福建省卫计委青年科研课题(2015-1-92)

摘　　要：目的考察余甘子醇提物体外对人胃癌AGS细胞的生长抑制和诱导凋亡的作用。方法采用MTT法、细胞集落实验、Hoechst染色分析、Annexin-V-FITC/PI等方法对经过余甘子醇提物体外作用的AGS细胞进行观察和检测。结果AGS细胞经0.05,0.1,0.15 mg·m L?1余甘子醇提物作用后,各组细胞形态均发生不同程度的变化,并呈现浓度-时间依赖性;细胞集落实验表明余甘子醇提物抑制肿瘤细胞的集落形成率;Hoechst染色分析、Annexin V/PI实验表明余甘子醇提物能够促进肿瘤细胞的凋亡。结论余甘子醇提物在体外对人胃癌AGS细胞具有良好的抑制生长和诱导凋亡的作用。OBJECTIVE To investigate the effects of ethanol extract of Phyllanthi Fructus on proliferation and apoptosis of human gastric cancer AGS cells in vitro. METHODS After the action of alcohol extract of Phyllanthi Fructus in vitro, the AGS cells were observed and detectd through MTT method, Cell colony experiment, Hoechst staining, and Annexin V/PI. RESULTS After the treatment with 0.05, 0.10, 0.15 mg·m L^-1 of alcohol extract, the morphology of AGS cells in each group changed different degrees and showed concentration-time dependence; Cell colony experiments showed that the ethanol extract of Phyllanthi Fructus can inhibit tumor cell colony formation rate; Hoechst staining analysis, Annexin-V-FITC/PI experiments showed that the ethanol extract of phyllanthi fructus can promote tumor cell apoptosis. CONCLUSION The alcohol extract of Phyllanthi Fructus in vitro has good inhibition activity and apoptosis induce on human gastric cancer AGS cells.

关 键 词：余甘子 人胃癌AGS细胞 抑制生长 诱导凋亡 

分 类 号：R285.5[医药卫生—中药学]