Bisphenol A accelerates capacitation-associated protein tyrosine phosphorylation of rat sperm by activating protein kinase A  

作　　者：Xiaofeng Wan Yanfei Ru Chen Chu Zimei Ni Yuchuan Zhou Shoulin Wang Zuomin Zhou Yonglian Zhang 

机构地区：[1]Shanghai Key Laboratory for Molecular Andrology, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [2]University of Chinese Academy of Sciences, Beijing 100864, China [3]State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China [4]State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China [5]Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China

出　　处：《Acta Biochimica et Biophysica Sinica》2016年第6期573-580,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Key Basic Research Program （Nos. 2014CB943103 and 2009CB941701） and the National Natural Science Foundation of China （Nos. 31471104 and 31171115）.

摘　　要：Bisphenol A （BPA） is a synthetic estrogen-mimic chemical. It has been shown to affect many reproductive endpoints. However, the effect of BPA on the mature sperm and the mechanism of its action are not clear yet. Here, our in vitro studies indicated that BPA could accelerate sperm capacitation-associated protein tyrosine phosphorylation in time- and dose-dependent manners. In vivo, the adult male rats exposed to a high dose of BPA could result in a significant increase in sperm activity. Further investigation demonstrated that BPA could accelerate capacitationassociated protein tyrosine phosphorylation even if sperm were incubated in medium devoid of BSA, HCO3^-, and Ca^2＋. However, this action of BPA stimulation could be blocked by H89, a highly selective blocker of protein kinase A （PKA）, but not by KH7, a specific inhibitor of adenylyl cyclase. These data suggest that BPA may activate PKA to affect sperm functions and male fertility.Bisphenol A （BPA） is a synthetic estrogen-mimic chemical. It has been shown to affect many reproductive endpoints. However, the effect of BPA on the mature sperm and the mechanism of its action are not clear yet. Here, our in vitro studies indicated that BPA could accelerate sperm capacitation-associated protein tyrosine phosphorylation in time- and dose-dependent manners. In vivo, the adult male rats exposed to a high dose of BPA could result in a significant increase in sperm activity. Further investigation demonstrated that BPA could accelerate capacitationassociated protein tyrosine phosphorylation even if sperm were incubated in medium devoid of BSA, HCO3^-, and Ca^2＋. However, this action of BPA stimulation could be blocked by H89, a highly selective blocker of protein kinase A （PKA）, but not by KH7, a specific inhibitor of adenylyl cyclase. These data suggest that BPA may activate PKA to affect sperm functions and male fertility.

关 键 词：bisphenol A SPERM tyrosine phosphorylation protein kinase A 

分 类 号：Q547[生物学—生物化学] S823.34[农业科学—畜牧学]