疏风解毒胶囊HPLC指纹图谱研究  

HPLC fingerprint of Shufeng Jiedu Capsule

作　　者：曹勇[1] 郭倩[2] 田成旺[3] 张铁军[3] 任涛[3] 李翔宇[1] 成娅婷 

机构地区：[1]安徽济人药业有限公司,安徽亳州236800 [2]天津医科大学,天津300070 [3]天津药物研究院,天津300193 [4]天津大学,天津300072

出　　处：《中草药》2016年第12期2034-2039,共6页Chinese Traditional and Herbal Drugs

摘　　要：目的建立疏风解毒胶囊(pgC)emiC指纹图谱分析方法,为全面有效控制其质量提供依据。方法采用emiC法建立pgC指纹图谱,色谱条件:rnitaryCNU色谱柱(ORMmm×QKSmm,Rμm),以乙腈JMKNB甲酸水溶液为流动相,梯度洗脱,体积流量NKMmiL min,柱温PM℃,检测波长ORMnm,进样量NMμi;对所建指纹图谱进行相似度分析及主成分分析(m CA),并对共有峰进行药材归属及成分指认。结果建立了pgCemiC指纹图谱共有模式,确定了OO个共有峰;NQ批pgC相似度在MKVNR^MKVTT,m CA表明前P个主成分代表了原始emiC指纹图谱的主要信息;在指纹图谱所标定的OO个共有峰中,U、NO、NP、NQ、NR、NS、ON、OO号峰来自虎杖,N、O、P、T、V、NM号峰来自连翘,R、S、NN号峰来自马鞭草,Q号峰来自败酱草,NU、NV号峰来自甘草,NT、OM号峰未能归属到具体药材;通过质谱数据比对,共指认出NS个共有峰,分别为N号峰(连翘酯苷b)、R号峰(戟叶马鞭草苷)、S号峰(马鞭草苷)、T号峰(R′J羟基连翘酯苷A)、U号峰(虎杖苷)、V号峰(连翘苷)、NM号峰(连翘酯苷A)、NN号峰(毛蕊花糖苷)、NO号峰(异连翘酯苷A)、NP号峰(芦荟大黄素)、NQ号峰(大黄素JUJlJ葡萄糖苷)、NR号峰(大黄酸)、NU号峰(甘草酸单铵盐)、NV号峰(PJ羟基光甘草酚)、ON号峰(大黄素)、OO号峰(大黄酚)。结论首次建立了pgCemiC指纹图谱分析方法,该法操作简单、准确,精密度、重复性好,可较全面地反映pgC中化学成分的信息,为pgC质量控制提供了可靠的科学依据。Objective To establish an HPLC method for the fingerprint analysis of Shufeng Jiedu Capsule （SJC）, so as to provide evidence for the quality control of it. Methods HPLC method was applied with the chromatographic condition as follows： The chromatographic column was Unitary C18 column （200 mm × 4.6 mm, 5 pm）, acetonitrile-0.1% formic acid as the mobile phase with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 250 nm, the column temperature was 30℃, and the injection volume was 10 μL. The HPLC fingerprint of SJC was analyzed with similarity analysis and principal component analysis （PCA） method. Results The fingerprint chromatography included 22 mutual peaks, of which eight mutual peaks from Polygonum cuspidatum, six mutual peaks from Forsythia suspensa, three mutual peaks from Verbena officinalis, two mutual peaks from Glycyrrhiza uralensis, and one mutual peak from Thlaspi arvense. The similarity among 14 batches was more than 0.9. Based on the HPLC-MS2 data, 16 components were identified, which were forsythosid E, hastatoside, verbenalin, 5＇-hydroxy-forsythiaside A, polydatin, phillyrin, forsythosid A, acteoside, iso-forsythosid A, aloe-emodin, emodin-8-O-grapeglycosides, parietic acid, monoammonium glycyrrhizinate, 3-hydroxyglabrol, emodin, and chrysophanol. Conclusion It is the first time to establish the HPLC fingerprint of SJC. The method is simple, accurate, and reproducible, which could reflect the chemical composition information of SJC comprehensively and provide scientific evidence for the quality control.

关 键 词：疏风解毒胶囊 HPLC 指纹图谱 质量控制 药材归属 成分指认 主成分分析 虎杖 连翘 马鞭草 败酱草 甘草 连翘酯苷E 戟叶马鞭草苷 马鞭草苷 5'-羟基连翘酯苷A 虎杖苷 连翘苷 连翘酯苷A 毛蕊花糖苷 异连翘酯苷A 芦荟大黄素 大黄素-8-O-葡萄糖苷 大黄酸 甘草酸单铵盐 3-羟基光甘草酚 大黄素 大黄酚 

分 类 号：R286.02[医药卫生—中药学]