基于液质联用技术的连翘化学成分分析  

Analysis on chemical constituents from Forsythiae Fructus by HPLC-Q-TOF-MS

作　　者：张晨曦[1,2] 刘素香[3] 赵艳敏[1,2] 张铁军[3] 刘岱琳 

机构地区：[1]天津中医药大学,天津300193 [2]中国人民武警警察部队后勤学院,天津300309 [3]天津药物研究院,天津300193

出　　处：《中草药》2016年第12期2053-2060,共8页Chinese Traditional and Herbal Drugs

摘　　要：目的 应用液质联用技术在正负离子模式下分析连翘Forsythiae Fructus中的主要化学成分。方法采用Agilent 1200HPLC,DiamonsilⅡC_（18）色谱柱（250mm×4.6mm,5μm）,以0.05%甲酸水溶液（A）-乙腈（B）为流动相,梯度洗脱,体积流量为1.0 mL/min,柱温35℃,进行200~600 nm全扫描,建立连翘指纹图谱,同时结合Broker Daltonics的1200HPLC-Q-TOF给出的正、负离子质谱信息和元素分析,与相关文献数据对照,分析化合物信息。结果结合HPLC-Q-TOF-MS提供的化合物准确相对分子质量,鉴定连翘中24个化合物,有12个苯乙醇苷类成分、7个木脂素类成分、5个黄酮类化合物,其中有5个化合物：3,4-二羟基苯乙醇苷（1）、2.甲氧基-3,4,5-三羟基苯乙醇苷（2）、β-羟基化连翘酯苷H（7）、6-羟基二氢黄酮（24）、二氢杨梅素（25）为首次从连翘中得到鉴定。结论为连翘药材的质量控制提供技术支持,为阐明连翘的药效物质基础提供数据参考。Objective To analysis the main chemical ingredients of Forsythiae Fructus by LC-MS technology in the positive and negative ions mode. Methods The chromatographic fingerprint was obtained with Diamonsil II C18 column （250 mm × 4.6 mm, 5 μm） and gradient elution with 0.05% H2O-formic acid （A）-acetonitrile （B）, and the flow rate was 1.0 mL/min. The column temperature was maintained at 35 ℃. The detection wavelength was 200-600 nm. Positive and negative ions MS information ofBruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with literature data to analyze the compounds information. Results Combined with the accurately relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 24 compounds were identified from the ethanolic extraction of Forsythia Fructus, which include 12 phenylethanoid glycosides, seven lignan ingredients, and five flavonoids. And 3,4-dihydroxyphenylethanoid glycoside （1）, 2-methoxy-3,4,5-trihydroxyphenylethanoid glycoside （2）, [3-hydroxyforsythoside H （7）, dihydroquercetagetin （24）, and dihydromyricetin （25） were first identified in Forsythiae fructus. Conclusion The method provides the technical support for the quality control of Forsythiae Fructus and contributes the reference data to elucidating the potential basis ofForsythiae Fructus.

关 键 词：连翘 液质联用技术 苯乙醇苷类 木脂素 黄酮 3 4-二羟基苯乙醇苷 β-羟基化连翘酯苷H 二氢杨梅素 

分 类 号：R284.1[医药卫生—中药学]