赶黄草水提浓缩液抗衰老作用及其机制研究  

Anti-aging effect and mechanism of concentrated solution of aqueous extract form Penthorum chinense

作　　者：刘中飞 刘明华 孙琴 税丕先 

机构地区：[1]西南医科大学药学院,四川泸州646000

出　　处：《中草药》2016年第13期2319-2323,共5页Chinese Traditional and Herbal Drugs

基　　金：四川省科技厅支撑计划"赶黄草GAP种植技术及其质量标准研究"(2014ZC0052);泸州市科技局重点项目"赶黄草新资源食品研究"(2013LZLY-K66)

摘　　要：目的研究赶黄草Penthorum chinense水提浓缩液抗衰老作用及其机制。方法以D-半乳糖制备亚急性衰老小鼠模型,采用小鼠避暗实验考察小鼠的学习记忆功能,测定脾脏指数、肝组织中单胺氧化酶(MAO)、血清中超氧化物歧化酶(SOD)及丙二醛(MDA)的水平,Western blotting检测脑组织中p53、p21蛋白的表达。结果赶黄草水提浓缩液能增强衰老模型小鼠的学习及记忆能力,增加脾脏指数,提高血清中SOD的活性,降低血清中MDA和肝脏中MAO的量,同时明显抑制脑组织中p53、p21蛋白表达,呈现一定的剂量依赖性。结论赶黄草水提浓缩液具有明显的抗衰老作用,其机制可能与其下调衰老信号通路p53/p21的蛋白表达、增强机体免疫功能、清除自由基有关。Objective To observe anti-aging effect and mechanism of concentrated solution of aqueous extract from Penthorum chinense. Methods Aging mice models were caused by D-galactose, learning and memory functions were investigated by avoid dark experiment, the spleen index, activity of MAO in liver, activities of SOD and MDA in serum were recorded, and the expression of p21 and p53 protein was detected by Western blotting. Results Concentrated solution of P. chinense by aqueous extraction increased the ability of learning and memory, improved spleen index and activity of SOD in serum, but it decreased the activity of MDA in serum and the activity of MAO in liver, and inhibited p21 and p53 protein expression in a dose-dependent manner. Conclusion Concentrated solution of aqueous extract from P. chinense could significantly exhibit anti-aging effects, its mechanism may be related to down-regulating the p53/p21 protein expression in aging signaling pathway, enhancing immune function, and removing free radicals.

关 键 词：赶黄草 抗衰老 D-半乳糖 超氧化物歧化酶 p53/p21 单胺氧化酶 

分 类 号：R285.5[医药卫生—中药学]