北细辛叶柄愈伤组织的增殖培养及器官分化研究  

Proliferation and organ differentiation of petiole-derived callus in Asarum heterotropoides

作　　者：何朋[1] 刘思妤[1] 王宏宇[1] 杨悦[1] 王丽娟[1] 吴秀菊[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《中草药》2016年第13期2341-2345,共5页Chinese Traditional and Herbal Drugs

基　　金：国家基础科学人才培养基金能力培养与科研训练项目(J1210069);黑龙江省教育厅科学技术研究项目计划(12531030);哈尔滨市科技局科技创新人才研究专项(2012RFLXN003)

摘　　要：目的研究北细辛Asarum heterotropoides叶柄愈伤组织增殖培养、再生芽分化及不定根诱导的最适条件,建立高效的愈伤组织培养和再生体系。方法以北细辛幼嫩叶柄诱导出愈伤组织,于含不同质量浓度6-苄基腺嘌呤(6-BA)和萘乙酸(NAA)的增殖培养基中扩大培养,选取嫩绿致密的愈伤组织接种在不同激素浓度配比的分化培养基上诱导再生芽及不定根,经驯化后移栽。结果愈伤组织增殖较适培养基为1/2 MS+0.40 mg/L 6-BA+0.10 mg/L NAA,在含0.40 mg/L 6-BA+0.05 mg/L NAA的1/2 MS培养基中再生芽分化率最高,无激素添加的1/2 MS培养基适于壮苗培养,不定根诱导最适培养基为1/4 MS+0.25 mg/L IBA。结论确定北细辛愈伤组织增殖及分化的最适培养基及激素水平,建立了完善的北细辛再生体系,成功获得再生植株。Objective To screen the optimum conditions of proliferation, regenerated bud differentiation, and adventitious roots induction of Asarum heterotropoides petiole-derived callus so as to establish the effective system for callus proliferation and regeneration. Methods Petiole-derived calli were subcultured in the proliferation medium supplemented with different concentration of 6-BA and NAA. Then green and compact calli were chosen to culture in differentiation medium with different exogenous hormones for the induction of regeneration bud and adventitious roots. After being acclimated, regenerated plants were transplanted. Results The effective callus proliferation medium was 1/2 MS supplemented with 0.40 mg/L 6-BA and 0.10 mg/L NAA, then 1/2 MS medium supplemented with 0.4 mg/L 6-BA and 0.05 mg/L NAA was suitable for regenerated bud differentiation while regenerated buds grew better in 1/2 MS medium without any hormone. The medium of 1/4 MS supplemented with 0.25 mg/L IBA was best for the induction of adventitious roots. Conclusion The optimum culture conditions of callus proliferation and differentiation in A. heterotropoides are determined to establish the regeneration system and obtain the regenerated plantlet successfully.

关 键 词：北细辛 愈伤组织增殖 再生芽分化 不定根诱导 再生体系 

分 类 号：R282.21[医药卫生—中药学]