珍稀濒危植物沙冬青的组织培养  

Tissue Culture of Endangered Psammophyte Ammopiptanthus mongolicus

作　　者：王方琳[1,2] 柴成武[1] 尉秋实[1] 魏小红[2] 马俊梅[1] 李爱德[1] 王昱淇 张莹花[1] 张锦春[1] 丁春发 赵颖[2] 

机构地区：[1]甘肃省治沙研究所/甘肃省荒漠化与风沙灾害防治重点实验室,甘肃武威733000 [2]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《中国农学通报》2016年第22期32-36,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"固定流沙先锋树种沙生柽柳无性繁殖生根机制研究"(31460069);国家自然科学基金项目"外源一氧化氮调控紫花苜蓿抗寒性的信号转导机制研究"(31560663);国家自然科学基金项目"风沙流对民勤荒漠灌木幼苗形态特征的影响及其生理生化响应机制"(31460224);国家自然科学基金项目"石羊河下游绿洲荒漠过渡带微区集水过程及其植被效应研究"(41161006);国家自然科学基金项目"石羊河中下游退耕地土壤系统演变规律及其驱动机制研究"(41161049)

摘　　要：试验以珍稀濒危植物沙冬青无菌苗的子叶、茎段为外植体材料,研究不同激素配比的培养基对不同外植体愈伤组织培养、丛生芽增殖及生根培养的影响,并筛选适宜各阶段培养的最佳培养基配方。结果表明,子叶是诱导沙冬青愈伤组织的最好外植体,在6-BA 0.5 mg/L、2,4-D 0.5 mg/L时,其愈伤组织诱导率达到最大值81.8%;在6-BA 2.0 mg/L、NAA 0.5 mg/L时丛生芽增殖率达到最大值90.2%,且丛生芽数量多、茎干健壮、生长旺盛,说明较高浓度的细胞分裂6-BA对丛生芽增殖具有明显的促进作用;在以1/2MS为基本培养基,加入NAA 1.0 mg/L时,生根率达到最大值70.4%,且生根时间最早,主根明显、粗壮且根毛数量多;适当调节培养基中无机盐的浓度提高生根率是将来沙生植物组织培养中研究的重点和难点。The paper aims to test effects of medium with different hormone combinations on explants callusculture, buds proliferation and rooting culture of rare and endangered plants Ammopiptanthus with asepticcotyledons and stem segments as explants materials, and screen the optimum medium formula for each culturestage. The results showed that cotyledon was the best explants to induct callus of Ammopiptanthus. The callusinduction rate reached its maximum 81.8% at 6- BA 0.5 mg/L, 2,4- D 0.5 mg/L. The clustered budsproliferation rate reached the maximum of 90.2% at 6- BA 2.0 mg/L, NAA 0.5 mg/L. The phenomenon ofmultiple clustered buds, robust stem and vigorous growth was observed, indicating that higher concentrations ofcell division 6-BA could significantly promote cluster bud proliferation. The rooting rate reached the maximumof 70.4% in 1/2MS basic medium added with NAA 1.0 mg/L, the rooting time was the earliest, with obviousmain root, which was stout and hairy. The key point in psammophytes tissue culture research will be adjustinginorganic salt concentration in the medium properly to improve the rooting rate.

关 键 词：沙冬青 组织培养 沙生植物 

分 类 号：Q94-331[生物学—植物学]