体外培养肠道菌群对白花蛇舌草黄酮提取物的代谢研究  

Study on the Metabolism of Hedyotis Diffusa Flavonoid Extract by Intestinal Bacteria in Vitro

作　　者：李芳[1,2] 杨培民[2] 曹广尚[2] 逯双 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药大学附属医院药学部,济南250011

出　　处：《医药导报》2016年第8期809-813,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(81274052);山东省自然科学基金资助项目(ZR2011HL043);山东省优秀中青年科学家科研奖励基金资助项目(BS2013YY052)

摘　　要：目的观察和比较人、正常大鼠、伪无菌大鼠肠道菌群对白花蛇舌草黄酮提取物的代谢作用。方法采用离体粪便温孵法,分别将白花蛇舌草提取物、芦丁单体、异槲皮苷单体、槲皮苷单体与人、正常大鼠及伪无菌大鼠肠菌孵育液在37℃厌氧培养,采用高效液相色谱法-二极管阵列检测器(HPLC-DAD)法对各组分进行定性分析,并根据各组分在不同温孵时间的色谱峰面积变化分析其代谢转化趋势。结果白花蛇舌草提取物中各组分及其单体容易被肠道菌群(人/正常大鼠)代谢,代谢速率很快,且主要代谢产物为槲皮素;在伪无菌大鼠肠菌孵育液中几乎没有代谢;肠道菌群对提取物中各组分代谢作用弱于相应单体。结论比较人、正常大鼠与伪无菌大鼠的代谢情况,进一步证实肠道菌群在黄酮类成分代谢中所起到的重要作用。Objective To study and compare the metabolism of Hedyotis diffusa flavonoid extract in intestinal flora from human, normal rats and pseudo germ-free rats in vitro. Methods Intestinal flora of human, normal rats and pseudo germ-free rats were incubated with Hedyotis diffusa extract, rutosid, isoquercitrin and quercitrin under anaerobic conditions at 37℃.High-performance liquid chromatography with diode-array detection （ HPLC-DAD ） was used for the analysis of components and metabolic products.The trends of metabolic transformation of the various components were analyzed according to the changes in chromatographic peak areas at different incubation time points. Results The components of Hedyotis diffusa extract and its monomer were quickly metabolized by human and normal rat intestinal flora and the main metabolite was quercetin.However, they were hardly metabolized by intestinal flora from pseudo germ-free rats. The metabolism effect of intestinal flora was weaker on components than on the corresponding monomers. Conclusion By comparing flavonoids metabolism in intestinal flora from human, normal rats and pseudo germ-free rats, the important role of intestinal microflora in the metabolism of flavonoids is further confirmed.

关 键 词：白花蛇舌草 芦丁 异槲皮苷 槲皮苷 槲皮素 肠道菌群代谢 

分 类 号：R285.5[医药卫生—中药学] R969.1[医药卫生—中医学]