一测多评法测定假马齿苋中间体中的5种三萜皂苷类成分含量  

Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker

作　　者：崔雨晴[1,2] 张继全[1,2] 吴飞[1,2,3] 阮克锋[1,2] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [2]上海张江中药现代制剂技术工程研究中心,上海201203 [3]上海中医药大学中药学院,上海201203

出　　处：《上海中医药杂志》2016年第7期88-93,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委资助项目(15DZ2292000);上海市科委科技支撑项目(15401902100)

摘　　要：目的利用一测多评法建立假马齿苋中间体中5种三萜皂苷类成分的高效液相色谱分析方法。方法以BacopasideⅠ为内参物,建立Bacoside A_3、BacopasideⅡ、Bacopasaponin C isomer、Bacopasaponin C的相对校正因子,从而计算各成分含量,并将一测多评结果与外标法实测值用相对误差评价,验证一测多评法的准确性。结果 BacopasideⅠ与Bacoside A_3、BacopasideⅡ、Bacopasaponin C isomer、Bacopasaponin C的相对校正因子分别为0.980、0.747、1.046、0.836,一测多评法计算结果与外标法无显著差异,相对误差小于5%。结论该方法准确可靠,简便可行,可作为假马齿苋中间体多指标成分测定质量控制方法。Objective To establish a quantitative analysis of muhi-components by single.-marker （QAMS） HPLC method for the determination of five triterpenoid saponins in the intermediates of Bacopa monnieri. Methods Bacopaside I was used as the internal marker to establish relative correction factor （RCF） between Bacopaside I and other four triterpenoid saponins, and RCF was used in the calculation of their content. Relative error was used in the appraisal of the result between external standard method and QAMS. Results The RCF between Bacopaside I and other four triterpenoid saponins were 0. 980, 0. 747, 1. 046 and 0. 836, respectively. The two methods did not show significant difference in assay results and the RCFs were reliable with the relative error less than 5%. Conclusion The established QAMS method is feasible, accurate, simple and can be used as a method for the determination of muhi-component index in the extract of Bacopa monnieri.

关 键 词：假马齿苋 一测多评法 相对校正因子 外标法 假马齿苋皂苷Ⅰ 苦艾素A_3 假马齿苋皂苷Ⅱ 假马齿苋皂苷C异构体 假马齿苋皂苷C 

分 类 号：R284[医药卫生—中药学]