汉防己甲素对系统性白念珠菌感染小鼠的治疗作用  

Tetrandrine on the dynamic changes of cytokines and antifungal effect in mice with Candida albicans

作　　者：石建萍[1] 李水秀[2,3] 朱坤举[2,3] 张玲玲[2,3] 张艺山[2,3] 张宏[2,3] 

机构地区：[1]广州医学院附属深圳沙井医院皮肤科,深圳518000 [2]暨南大学附属第一医院 [3]暨南大学真菌病研究所,广州510632

出　　处：《中国人兽共患病学报》2016年第8期689-695,716,共8页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81171542/81471995);广东省医学科研基金(No.A2016377)联合资助~~

摘　　要：目的观察汉防己甲素在系统性白念珠菌感染小鼠中抗真菌疗效、细胞免疫调节效应及两者相关性。方法雌性BALB/c小鼠随机分为对照组和感染组。对照组予生理盐水,感染组在尾静脉注射白念珠菌悬液后3h,经腹腔注射不同剂量汉防己甲素(0、15、30、45和90mg/kg),每日一次,连续7d。观察小鼠生存时间、肾脏载菌量以及肾脏组织病理学检查评估抗真菌疗效;同时以ELISA法检测血清和Real-time RT-PCR检测肾组织中细胞因子IFN-γ、IL-10、TGF-β1和IL-17A水平的动态变化。结果与腹腔注射生理盐水的感染小鼠比较,汉防己甲素在90mg/kg时,肾脏载菌量减少(P<0.05),与升高血清及肾组织IFN-γ、TGF-β1水平,降低IL-10、IL-17A水平有相关性;在45mg/kg及以下剂量时,生存时间、组织载菌量及细胞因子无差异(P>0.05)。结论汉防己甲素在90mg/kg时对系统性白念珠菌感染小鼠具有治疗作用,与其细胞免疫调节有相关性。To study the therapeutic effect of tetrandrine（TET）on the mice infected with Candida albicans.The mice with disseminated candidiasis were randomly divided into experimental group and control group,The serum level of the IFN-γ,IL-10,TGF-β1and IL-17 A were determined by ELISA and kidney IFN-γ,IL-10,TGF-β1and IL-17 A mRNA expression levels were tested with real-time RT-PCR method.At the same time,kidmey fungal burdens were measured.Results showed that therapy with TET ranging from 15 to 45mg/kg did not affect organ fungal burdens and survival times（P〉0.05）,and has nothing with the cellular immunity.However,90mg/kg of TET decreased organ fungal burdens and prolonged survival times,and correlated with increasing levels of cytokines of IFN-γand TGF-β1,decreasing levels of IL-17 Aand IL-10 when observed in mice infected with SC5 314 compared with control group on day 8after infection.Thus,90mg/kg of TET has therapeutic effects on disseminated candidiasis of mice.

关 键 词：汉防己甲素 白念珠菌 小鼠 

分 类 号：R378[医药卫生—病原生物学]