反相HPLC法同时测定大麻植物中的三种有效成分  

Simultaneous Determination of Three Kinds of Effective Constituents in Cannabis Plants by Reversed-phase HPLC

作　　者：傅强[1] 舒智 邓轲[1] 罗璇[1] 曾昌广 

机构地区：[1]德阳市公安局刑警支队,四川德阳618000 [2]临沧市公安局,云南临沧677000

出　　处：《法医学杂志》2016年第4期261-263,共3页Journal of Forensic Medicine

摘　　要：目的建立同时测定大麻植物中四氢大麻酚(tetrahydrocannabinol,THC)、大麻二酚(cannabidiol,CBD)和大麻酚(cannabinol,CBN)三种有效成分的高效液相色谱(HPLC)法。方法采用通用C_(18)色谱柱,以乙腈-磷酸盐缓冲液(0.015 mol/L KH_2PO_4)为流动相,流速为1.0 m L/min,检测波长为220 nm,同时收集波长190~400nm的紫外光谱图,并以此光谱图及保留时间作为定性依据。结果所建方法能良好地分离THC、CBD和CBN,三种成分在0.4~40μg/m L范围内线性关系良好(R^2≥0.999 3),回收率大于87%,最低检出限分别为1.8、2.0和1.3 ng,日内精密度与日间精密度均小于5%。结论反相HPLC法简便、快速、准确,适用于大麻植物中THC、CBD和CBN的定性定量检测。Objective To establish a high performance liquid chromatographic （HPLC） method for simultaneous determination of three effective constituents, including tetrahydrocannabinol （THC）, cannabidiol （CBD） and carmabinol （CBN） in Cannabis plants. Methods A C18 column was used in this study, and acetonitrile-phosphate buffer （0.015 mol/L KH2PO4） was used as mobile phase at a flow rate of 1.0mL/min. At a detection wavelength of 220 mm, UV absorption spectra were collected at the wavelength range of 190-400 nm, and the spectra and retention time were counted as qualitative evidence. Results THC, CBD and CBN could be well separated by this method. Three components had good linear relationship in the range of 0.4-40μg/mL （RZ≥0.999 3）. The recoveries were over 87%. The limits of detection were 1.8ng, 2.0rig and 1.3ng, respectively. The relative standard deviation （RSD） were less than 5% for both inter-day and intra-day precisions. Conclusion Reversed-phase HPLC method is simple, rapid and accurate, and it is suitable for the qualitative and quantitative detection of THC, CBD and CBN in Cannabis plants.

关 键 词：法医毒理学 高效液相色谱 大麻属 四氢大麻酚 大麻二酚 大麻酚 

分 类 号：R286.0[医药卫生—中药学]