MC1R蛋白在不同毛色太行山羊皮肤组织中的表达定位  

Expression and Localization of MC1R in the Skin Tissues of Taihang Goats with Different Coat Colors

作　　者：景炅婕[1] 潘洋洋[1] 张莹姣 马元[1] 乔利英[1] 刘建华[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《畜牧与饲料科学》2016年第8期5-8,12,共5页Animal Husbandry and Feed Science

基　　金：山西省研究生优秀创新项目(2015SY27);山西省科技攻关项目(20120312010);科技部863子课题(2011AA100307-05)

摘　　要：MC1R基因是调节哺乳动物毛色形成的重要候选基因之一,编码黑色素皮质素受体1(MC1R),在哺乳动物黑色素的代谢中发挥重要作用。分别选取纯黑色、纯白色、黄褐色和黑斑白色的太行山羊皮肤组织,通过HE染色技术,观察太行山羊的皮肤组织结构;利用免疫组化技术,对MC1R蛋白在4种毛色太行山羊皮肤组织中的表达情况进行研究。结果表明,太行山羊皮肤由表皮、真皮、皮下结缔组织3部分组成;MC1R蛋白在4种毛色太行山羊的表皮、真皮中均没有表达,但在纯黑、黄褐和黑斑白色太行山羊毛囊中有表达,且主要集中在毛囊外根鞘处。研究结果为太行山羊进一步的提纯选育工作提供了一定的依据。MC1R is one of the important candidate genes that are associated with the regulation of mammalian coat colors. Melanocortin 1 receptor （MC1R） is encoded by this gene which plays important roles in the metabolism of mammalian melanin. In the present study, the structures of skin from the black, white, brown and white with black-spots Taihang Goats were observed using the HE staining method, respectively. Immunohistochemistry assay was performed to study the expression of MC1R in the skins of goats with different coat colors. Results showed that the skins of the Taihang Goats consisted of epidermis, subdermal and subcutaneous connective tissue. No expression MC1R of was found in epidermis and subdermal in the tissues from goats with different coat colors. However, the expression MC1R was observed in the hair follicles of the black, brown and white with black- spots Taihang Goats which was mainly distributed in the outer root sheath. The results obtained in this study provide a basis for the further genetic selection of Taihang Goats.

关 键 词：太行山羊 皮肤组织 MC1R HE染色 免疫组织化学技术 

分 类 号：S827.952[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]