北极海洋放线菌Streptomyces sp.604F中的抗霉素A和伏尔加霉素类成分  

ANTIMYCIN A AND VULGAMYCIN DERIVATIVES FROM THE ARCTIC MARINE ACTINOMYCETE STREPTOMYCES SP.604F

作　　者：江昇平 田晓清[1] 廖丽[3] 杨桥[1] 陆亚男[1] 马丽艳[1] 陈波[3] 樊成奇[1] 

机构地区：[1]农业部东海与远洋渔业资源开发利用重点实验室,中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学,上海201306 [3]中国极地研究中心,上海200136

出　　处：《极地研究》2016年第3期331-335,共5页Chinese Journal of Polar Research

基　　金：国家自然科学青年基金(41406181);国家高技术研究发展计划项目(2012AA092105);国家海洋局“南北极环境综合考察与评估”专项(CHINARE 2015-04-03);上海市自然科学基金(13ZR1462700)资助

摘　　要：极地微生物代谢产生芳香族硝基化合物、肽类、萜类等多种骨架类型的化学成分。为从北极放线菌中发现结构新颖、活性显著的小分子化合物,本研究采用Sephadex LH-20凝胶和半制备HPLC等柱层析分离纯化化合物手段,结合LC-MS、1H-和13C-NMR等波谱学数据和文献对比进行结构解析,从链霉菌Streptomyces sp.604F提取物中分离鉴定出抗霉素A1b(1)、抗霉素A4a(2)、抗霉素A2a(3)、抗霉素A3a(4)、抗霉素A1a(5)以及伏尔加霉素(6)和8-脱氧伏尔加霉素(7)这7个化合物。化合物1—7是首次从北极海洋沉积物来源的微生物中发现,而且是首次从同一菌株中发现抗霉素A和伏尔加霉素这两类化合物。Various types of constituents,including aromatic nitro compounds,peptides,and terpenoids,have been reported from different polar microorganisms. In our investigation of secondary metabolites of Arctic actinomycete with new structures or potent bioactivity,five antimycin A derivatives,Antimycin A1b（ 1）,Antimycin A4a（ 2）,Antimycin A2a（ 3）,Antimycin A3a（ 4）,Antimycin A1a（ 5）,together with Vulgamycin（ 6） and its derivative 8-deoxyenterocin（ 7）,were obtained from Streptomyces sp. 604 F. The compounds were purified by Sephadex LH-20 column chromatography and semi-preparative high-performance liquid chromatography,and their structures were determined by comparing their liquid chromatograph-mass spectrometry and1H- and13C-NMR data with those of previously reported compounds. This is the first report of compounds 1-7 from microbes derived from Arctic sediment,and it is the first time that antimycin and vulgamycin compounds were identified as metabolites from the same source.

关 键 词：北极海洋放线菌 链霉菌604F 抗霉素A 伏尔加霉素 

分 类 号：Q936[生物学—微生物学]