清化颗粒对db/db糖尿病小鼠肠道苦味受体的影响  

Effects of Qing-Hua Granule on Bitter Taste Receptors in Ileum of db / db Diabetes Mice

作　　者：李俊燕[1] 王静懿[1] 徐杰[1] 陆灏[1] 陶枫[1] 

机构地区：[1]上海中医药大学附属曙光医院糖尿病研究所内分泌科,上海201203

出　　处：《上海中医药大学学报》2016年第4期47-51,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81403359);中国博士后科学基金项目(2015M581653);上海市中医药事业发展三年行动计划项目(ZY3-CCCX-2-1003);上海市自然科学基金项目(13ZR1442700)

摘　　要：目的:探讨清化颗粒对db/db糖尿病小鼠肠道苦味受体(taste receptor type 2,TAS2Rs)的影响。方法:将db/db糖尿病小鼠随机分为模型对照组及清化颗粒低、中、高剂量组,db/m小鼠为空白对照组。清化颗粒低、中、高剂量组分别灌胃给药(3.77、7.54、15.08 g·kg-1·d-1),模型对照组和空白对照组灌胃等体积的生理盐水,均干预4周。干预结束后,腹腔注射葡萄糖耐量试验(IPGTT)观察每组小鼠血糖水平变化;ELISA法分析小鼠血清胰高血糖素样肽-1(GLP-1)浓度;qRT-PCR测定每组小鼠肠道苦味受体(TAS2R7、TAS2R9和TAS2R38)的mRNA水平;Western blot检测小鼠肠道苦味信号通路因子磷酸二酯酶1A(PDE1A)的蛋白水平。结果:清化颗粒各剂量组的空腹血糖水平均较模型对照组显著降低(P<0.01),血清GLP-1浓度显著高于模型对照组(P<0.01);清化颗粒中、高剂量组的TAS2R7、TAS2R9和TAS2R38的mRNA水平较模型对照组显著增加(P<0.01);清化颗粒各剂量组的PDE1A的蛋白水平较模型对照组显著增加(P<0.01),呈剂量依赖效应。结论:清化颗粒能够降低db/db糖尿病小鼠的空腹血糖水平,其机制可能与促进肠道GLP-1分泌的苦味信号通路有关。Objective： To discuss the effect of Qing-Hua granule（ QHG） on taste receptor type 2（ TAS2Rs） in ileum of db / db diabetes mice. Methods： The db / m mice were set as normal control group. The db / db diabetes mice were randomized into the model control group, QHG group with low-, middle-and high-dose. QHG groups with different dose were intragastrically administrated with 3. 77、7. 54、15. 08 g · kg-1·d-1respectively,and the model control group and normal control group were administrated with normal saline of equal volume. All the groups were treated for 28 days. The blood glucose was detected by IPGTT. The concentration of glucagon-like peptide-1（ GLP-1） in serum was detected by ELISA. The mRNA levels of bitter taste receptors（ TAS2R7,TAS2R9 and TAS2R38） were detected by qRT-PCR. The protein expression of phosphodiesterase 1A（ PDE1A） was examined by Western blot. Results： Compared with the model control group,the levels of fasting blood glucose（ FBG） in each dose group of Qinghua granules decreased significantly（ P 〈 0. 01）; the mRNA levels of TAS2R7,TAS2R9 and TAS2R38 in each dose group of Qinghua granules increased significantly（ P 〈 0. 01）; the protein expression of PDE1 A in each dose group of Qinghua granules increased significantly（ P 〈 0. 01） in a dose-dependent manner.Conclusion： QHG could significantly reduce the FBG level of db / db mice through the bitter signaling pathway secreted by intestinal GLP-1

关 键 词：清化颗粒 DB/DB糖尿病小鼠 苦味受体 磷酸二酯酶1A 

分 类 号：R285.5[医药卫生—中药学]