HPLC法测定紫苏叶中咖啡酸、野黄芩苷和迷迭香酸的含量  

Determination of Caffeic Acid,Scutellarin and Rosmarinic Acid in Perillae Folium by HPLC

作　　者：胡惠军[1] 谷丽华[1] 侴桂新[1] 

机构地区：[1]上海中医药大学中药研究所上海中药标准化研究中心,上海201203

出　　处：《上海中医药大学学报》2016年第4期80-84,共5页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家中医药管理局中医药行业科研专项项目(201307002)

摘　　要：目的:建立同时测定紫苏叶中咖啡酸、野黄芩苷和迷迭香酸的HPLC方法。方法:采用Fortis Xi C18色谱柱(250 mm×4.0 mm,5μm),以乙腈-0.2%冰醋酸为流动相进行梯度洗脱,柱温为25℃,体积流量为1 ml/min,检测波长330 nm,进样量10μl。结果:3个被测成分在各自测定的线性范围内线性良好,r≥0.999 9,加样回收率范围分别为97.74%、98.76%、101.5%,RSD分别为1.7%、3.1%和2.3%。结论:所建立的方法操作简单,稳定性和重复性好,可用于紫苏叶药材中咖啡酸、野黄芩苷和迷迭香酸含量的测定。Objective： To measure the contents of caffeic acid,scutellarin and rosmarinic acid in Perillae Folium by HPLC. Methods： Fortis Xi C18column（ 250 mm × 4. 0 mm,5 μm） was used. The mobile phase consisted of acetonitrile and water solution with 0. 2% acetic acid was used as gradient elution for separation. The column temperature was 25 ℃. The flowing rate was 1 ml·min-1. The detecting wavelength was at 330 nm and the inject volume was 10 μl. Results： It showed good linearities in the measuring range of the three tested constitutions,r≥0. 999 9. The ranges of recoveries were 97. 74%,98. 76% and 101. 5% respectively,and the corresponding RSD were 1. 7%,3. 1% and 2. 3%. Conclusion： The established method shows simple operation,good stability and repeatability,which can be applied in the determination of caffeic acid,scutellarin and rosmarinic acid of Perillae Folium.

关 键 词：紫苏叶 含量测定 HPLC 咖啡酸 野黄芩苷 迷迭香酸 

分 类 号：R284.1[医药卫生—中药学]