安徽省中北部淡色库蚊对多种杀虫剂抗性及其kdr基因突变研究  

Multiple insecticide resistances and kdr mutation of Culex pipiens pallens in north-central Anhui Province

作　　者：魏星[1,2] 杨小迪[1] 王小莉[1] 李江艳[1] 王媛媛[1] 夏惠[1] 常雪莲[1] 方强[1] 

机构地区：[1]蚌埠医学院病原生物学教研室、安徽省感染与免疫重点实验室,蚌埠233030 [2]蚌埠医学院公共课程部

出　　处：《中国血吸虫病防治杂志》2016年第4期406-410,共5页Chinese Journal of Schistosomiasis Control

基　　金：安徽省高校自然科学研究重点项目(KJ2015A146;KJ2016A708);蚌埠医学院自然科学重点项目(BYKY1403ZD);安徽省高校省学术技术带头人培养资助项目(2014053);安徽高校科研创新平台团队项目(2016-40)1 2330302

摘　　要：目的了解安徽省中北部地区淡色库蚊对多种杀虫剂的抗药性现状及其kdr基因突变情况。方法 2014年6-9月在淮北市、蚌埠市和滁州市3地采集淡色库蚊幼虫,带回实验室饲养至成虫,采用WHO成蚊接触筒药膜滤纸接触法对其进行0.05%溴氰菊酯、5%马拉硫磷、0.1%噁虫威、4%DDT抗性生物测定。以PCR扩增经溴氰菊酯抗性测定的库蚊抗性相关kdr基因并进行测序,统计L1014位点突变情况。结果上述3个地区淡色库蚊对溴氰菊酯、马拉硫磷、噁虫威、DDT 4种杀虫剂都产生了不同程度的抗药性,对DDT产生的抗性较高;虽然3地淡色库蚊接触DDT后的死亡率差异无统计学意义(F=1.027,P>0.05);但接触溴氰菊酯、马拉硫磷、噁虫威后的死亡率差异均有统计学意义(F=23.823、33.955、128.841,P均<0.01)。3地淡色库蚊种群的kdr基因1014位点均存在L1014F、L1014S这2种非同义突变;L1014F突变频率与溴氰菊酯抗性水平呈正相关(r2=0.718,P<0.01)。结论安徽省中北部地区淡色库蚊对溴氰菊酯、马拉硫磷、噁虫威、DDT均产生了较强的抗性,kdr基因L1014F突变频率与溴氰菊酯抗性水平呈正相关;各地区卫生部门需加强对媒介蚊虫抗性的动态监测。Objective To understand the status of resistance to multiple insecticides and the frequencies of kdr mutations in Culex pipiens pallens from northcentral Anhui Province. Methods From July to September,2014,the C. pipiens pallens mosquito larvae were collected in Huaibei,Bengbu and Chuzhou cities of the northcentral Anhui Province and reared to adults. The female adult mosquitoes at 3-5 days post emergence were tested for susceptibility to the four insecticides,namely 0.05%deltamethrin,5%malathion,0.1%bendiocarb and 4%DDT,by using the standard WHO resistance tube bioassay. The detection of the point mutations of the kdr gene at codon 1014 was conducted by PCR and DNA sequencing in the deltamethrinresistant andsusceptible mosquitoes. Results High levels of resistance to all the four insecticides were found in all the three tested populations,although mosquito mortality varied among populations and test insecticides. Among the test insecticides,DDT showed lowest mortality with no significant difference（F=1.027,P〉0.05）in all test populations,whereas significantly different mortalities were observed among populations for the remained three insecticides tested（deltamethrin,malathion,and bendiocarb）（F = 23.823,33.955,128.841;all P〈0.01）. Two types of nonsynonymous kdr mutation at codon position 1014 （L1014F and L1014S）were observed. A positive correlation between L1014F mutation frequencies and deltamethrin resistance levels were detected in the three mosquito populations（r2=0.718,P〈0.01）. Conclusions The observed high levels of resistance to multipleinsecticides coupled with the occurrence of medium to high kdr frequencies in populations of C. pipiens pallens could profoundly affect the mosquito vector control programme in China. The local health departments need to strengthen vector＆amp;nbsp;dynamic monitoring and implement rational resistance management strategies.

关 键 词：淡色库蚊 杀虫剂抗药性 溴氰菊酯 马拉硫磷 噁虫威 kdr基因突变 安徽省 

分 类 号：R184[医药卫生—流行病学]