CD4^＋CD25^＋FoxP3^＋Treg细胞在儿童重症肺炎支原体肺炎中的作用  

CD4^＋CD25^＋FoxP3^＋ Treg in the immune mechanism of severe mycoplasma pneumoniae pneumonia in children

作　　者：郭赟[1] 钱俊[1] 李羚[1] 惠郁[1] 蒋汉民[1] 

机构地区：[1]南京医科大学附属无锡人民医院儿童医院呼吸科,214023

出　　处：《国际儿科学杂志》2016年第8期647-650,共4页International Journal of Pediatrics

摘　　要：目的探讨CD4^＋CD25^＋FoxP3^＋Treg细胞在儿童重症肺炎支原体肺炎（severe mycoplasma pneumoniae pneumonia, SMPP）中的作用。方法采用流式细胞仪检测65例重症肺炎支原体肺炎患儿（SMPP组）、75例非重症肺炎支原体肺炎患儿（Non—SMPP组）及40例健康儿童（健康对照组）急性期及恢复期外周血CD4^＋CD25^＋FoxP3^＋Treg细胞占CD4^＋T细胞的比例并进行比较分析。结果急性期SMPP组CD4^＋CD25^＋FoxP3^＋Treg表达低于Non—SMPP组（0．87±0．66％vs．1．17±0．70％，P〈0．05）及对照组（0．87±0．66％vs．3．88±2．00％，P〈0．01）。Non—SMPP组CD4^＋CD25^＋FoxP3^＋Treg表达低于对照组（I．17±0．70％VS．3．88±2．00％，P〈0．01）。恢复期SMPP组CD4^＋CD25^＋FoxP3^＋淋巴细胞比例较Non-SMPP组降低（1．66±0．85％vs．3．61±1．45％，P〈0．01）。恢复期SMPP组CD4^＋CD25^＋FoxP3^＋淋巴细胞比例较急性期升高（1．66±0．85％vs．0．87±0．66％，P〈0．01），恢复期Non—SMPP组CD4^＋CD25^＋FoxP3^＋淋巴细胞比例较急性期亦升高（3．61±1．45％VS．1．17±0．70％，P〈0．01）。结论CD4^＋CD25^＋FoxP3^＋Treg在SMPP的发病中起一定作用，低表达CD4^＋CD25^＋FoxP3^＋Treg的患儿可能在感染MP后患SMPP的易患性增加，同时影响SMPP患儿预后。Objective To investigate the role of CD4^＋CD25^＋FoxP3^＋ in severe Mycoplasma pneumonia among children. Methods One hundred and forty children with M. pneumoniae pneumonia （65 severe and 75 non-severe） who were hospitalized were enrolled along with forty other children as controls. X-ray was assessed. The proportions of peripheral blood CD4^＋CD25^＋FoxP3^＋ cells were determined by flow cytometry. Results Both severe and non-severe children had decreased CD4^＋CD25^＋FoxP3^＋ cells as compared with control subjects in acute phase （0.87±0.66% vs. 3.88 ±2.00%, P〈0.01 and 1.17 ±0.70% vs. 3.88 ± 2. 00%, P 〈0. 01, respectively）. The levels of CD4^＋CD25^＋FoxP3^＋ cells in severe children were lower than those in non-severe children in acute phase and recovery phase （0. 87± 0. 66% vs. 1.17 ± 0.70%, P 〈 0. 05 and 1.66±0. 85% vs. 3.61± 1.45%, P 〈0. 01, respectively） . Both severe children and non-severe children expressed higher CD4^＋CD25^＋FoxP3^＋ cells in recovery phase than in acute phase （ 1.66 ± 0. 85% vs. 0. 87± 0.66%, P〈0.01 and 3.61±1.45% vs. 1.17±0.70%, P〈0.01, respectively）. Conclusion The expression of CD4^＋CD25^＋FoxP3^＋ Tregs may play a role in the onset of severity of mycoplasma pneumonia and the low express of CD4^＋CD25^＋FoxP3^＋ Tregs in children infected with M. pneumonia may increase the susceptibility to severe mycoplasma pneumonia.

关 键 词：调节T细胞 肺炎支原体肺炎 重症 免疫机制 

分 类 号：R725.6[医药卫生—儿科]