Chop及Grp78基因在先天性脊柱裂大鼠胚胎12天神经管中的异常表达  

Altered expression of Chop and Grp78 in neural tube of rat with spina bifida

作　　者：吴迪[1] 刘波[1] 范洋[1] 

机构地区：[1]中国医科大学附属盛京医院研发中心,沈阳110004

出　　处：《国际儿科学杂志》2016年第8期651-653,共3页International Journal of Pediatrics

基　　金：国家自然科学基金青年项目（81200481）

摘　　要：目的探讨Chop及Grp78基因在先天性脊柱裂大鼠胚胎12d神经管及神经上皮干细胞中的表达情况。方法取畸形大鼠12d胚胎神经管组织，荧光实时定量PCR考察Chop及Grp78基因表达。另取12d正常大鼠胚胎，分离消化神经管行神经上皮干细胞培养。荧光实时定量PCR考察Chop及Grp78基因表达。结果先天性脊柱裂大鼠胚胎12d神经管内Chop基因及Grp78基因表达异常增高。神经上皮干细胞分化为神经元后Chop及Grp78基因的表达上调。结论Chop及Grp78基因在先天性脊柱裂大鼠胚胎12d神经管中表达异常增高，二者在神经上皮干细胞分化为神经元后表达上调，可能与内质网应激相关的凋亡有关。神经上皮干细胞培养培养体系稳定、高效，可以为胚胎早期发育提供实验模型。Chop and Grp78 genes are key genes in endoplasmic reticulum stress（ERS）. To explore the gene alteration of Chop and Grp78 in embryo with spina bifida,we studied Chop and Grp78 gene expression by qRT-PCR in neural tube of rat with spina bifida in E12 and primary cultured neuroepithelial stem cells（NEP）. We found higher expression of both Chop and Grp78 in neural tube of rat with spina bifida than normal rats. While Chop and Grp78 genes were up-regulated after NEP differentiated into neuron. The up-regulation of Chop and Grp78 genes in neural tube of rat and NEP might be involved in the abnormal apoptosis in spina bifida related with ERS. And we found the primary culture of NEP provided a good model for embryo early development research.

关 键 词：先天性脊柱裂 Chop基因 Grp78基因 神经上皮干细胞 

分 类 号：R726.8[医药卫生—儿科]