甜玉米胚乳突变基因shrunken2的序列变异解析  

Analysis of Sequence Polymorphism of shrunken2 in Sweet Corn

作　　者：索海翠[1,2,3] 郑锦荣[2,3] 张小兰[2,3] 李智军[2,3] 李武[2,3] 胡建广[2,3] 韩福光[2,3] 王科翰[2,3] 王丽[2,3] 李余良[2,3] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]广东省农业科学院作物研究所,广州510640 [3]广东省作物遗传育种重点实验室,广州510640

出　　处：《分子植物育种》2016年第5期1049-1058,共10页Molecular Plant Breeding

基　　金：广东省科学技术厅广东省农作物遗传改良重点实验室建设项目(粤科财字(2013)82号);广东省农业科技研究团队项目(2011A020102010);广东省科技计划项目(2009B080701103;2011A030100002;2014A020208061;2016A020208006)共同资助

摘　　要：Shrunken2基因编码葡萄糖焦磷酸化酶的大亚基,是玉米淀粉合成途径中的限速酶,shrunken2是Shrunken2的隐性突变等位基因,对甜玉米品质和风味的形成起着重要的作用。本研究以玉米B73的Shrunken2基因为参考序列,在58份甜玉米自交系中进行shrunken2基因的重测序,共获得了7 635 bp的核苷酸序列,涵盖了20个外显子,17个内含子以及3'UTR区域。基因多态性分析发现,在供试群体中共有多态性位点105个,包括78个SNP和27个Indel。与普通玉米B73的Shrunken2序列之间存在58个多态性位点差异,包括50个SNP和8个Indel,其中位于编码区的SNP位点包含2个同义突变和7个非同义突变。根据shrunken2基因全长序列和编码区序列可将该群体分成14种和4种单倍型。中性检测表明该基因经历了自然和人工双重选择过程。Shrunken2 encodes the large subunits of ADP-glucose pyrophosphorylase （AGPase）, which catalyzes the rate limiting step in the process of starch biosynthesis in maize. While shrunken2 is the recessive mutant of Shrunken2, it plays an important role in formation of flavor and quality in sweet corn. In this research, we re-sequenced shrunken2 gene in 58 sweet corn inbred lines according to the sequence of Shrunken2 in B73. Total 7 635 bp sequence was obtained, which covered 20 exons （from exon 1 to exon 20）, 17 introns （from intron 1 to intron 19, except intron 2 and intron 17） and 3＇UTR region. From the analysis of gene polymorphism, a total of 105 polymorphism sites were identified, including 78 SNPs and 27 Indels. There were 58 polymorphism sites differences in shrunken2 sequences compared with that in Shrunken2 in B73, including 50 SNPs and 8 indels. Besides, 2 synonymous and 7 non-synonymous SNP mutations were found in the shrunken2 cording region. According to the shrunken2 gene sequence and coding region sequence, the inbred lines could be respectively divided into 14 and 4 haplotypes, Neutral evolution test showed that the shrunken2 gene was experienced both natural and artificial selection.

关 键 词：甜玉米 Shrunken2 序列多态性 中性检测 单倍型 

分 类 号：S513[农业科学—作物学]