星星草体细胞无性系遗传稳定性分析  

Genetic Stability Analysis of Somaclones from Alkali Grass(Puccinellia Tenuiflora)

作　　者：李晓玲[1,2] 于晓明[2] 房强[1] 孙倩文[2] 

机构地区：[1]长春工业大学化学与生命科学学院,长春130012 [2]东北师范大学生命科学学院,长春130024

出　　处：《分子植物育种》2016年第5期1236-1243,共8页Molecular Plant Breeding

基　　金：吉林省自然科学基金项目(201115155);长春工业大学自然科学基金项目(2007103)共同资助

摘　　要：本研究以星星草(Puccinellia tenuiflora)种子在愈伤组织诱导培养基上萌发长出的纤细幼苗为亲本对照,分别应用AFLP和S-SAP技术,对其诱导的愈伤组织及其体外再生植株等体细胞无性系的遗传稳定性进行了分析。结果表明,以星星草亲本对照和随机选取的同起源的3瓶愈伤组织、8株星星草再生苗基因组DNA为模板,分别应用19对AFLP引物进行选择性扩增反应,结果表明,在电泳图谱上共检测到925条DNA条带,其中16条DNA片段呈多态性,遗传变异频率为1.7%;同时,在上述分析样品中,应用24对S-SAP选择性扩增引物共扩增出的789条DNA片段,其中36个位点具有多态性,变异频率达到了4.6%。聚类分析结果表明基于AFLP和S-SAP数据分析的样本彼此间平均遗传相似性系数分别为0.995和为0.987。In this study, we used the slender shoots that grew up from a seed ofP. tenuiflora on the callus-inductionmedium as donor control, and analyzed the genetic stability of somaclones of induced callus and regenerated plants in vitro were analyzed by AFLP and S-SAP methods respectively. We used the genomic DNA of donor control, three randomly chosen bottles of callus and eight randomly chosen regenerated plants derived from the same resource as template, and performed selective amplification by 19 pairs of AFLP primer respectively. The results showed that 925 DNA bands were detected in the electrophoresis patterns and 16 DNA bands were polymorphic, genetic variation frequency was 1.7%. In addition, 789 DNA bands were amplified by 24 pairs of S-SAP selective amplification primer in the above analysis samples, 36 sites were polymorphic, genetic variation frequency was 4.6%. The result of cluster analyses showed that the average genetic similarity coefficient of the samples were 0.995 and 0.987 respectively basing on AFLP and S-SAP data analysis.

关 键 词：星星草 体细胞无性系 遗传稳定性 分子标记 

分 类 号：S543.9[农业科学—作物学]