原核表达GST-GAPDH蛋白的快速斑点印渍检测  

Rapid dot blot method to detect the GST-GAPDH protein expressed in prokaryotic systems

作　　者：石建党[1] 李小菊[1] 张宝珠[1] 李欣[1] 赵玉红[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《实验室科学》2016年第3期30-33,共4页Laboratory Science

基　　金：国家基础科学人才培养基金项目(项目编号:J1103503);南开大学教改项目"新型分子生物学实验教学体系的构建"

摘　　要：western blot是生命科学研究中分析特定蛋白质的常用手段。在分子生物学实验课程已经建立的克隆小鼠GAPDH基因编码序列教学过程的基础上,以IPTG诱导后的原核细胞裂解液为实验材料,建立了适合课题教学的快速斑点印渍检测方法,使学生能够很好地掌握western blot的实验原理和方法;直观地观察到自己整个分子克隆实验的成果,激发了学生的学习兴趣,促进了学生的科学研究能力的提升。The western blot is a widely used method to detect specific proteins in a sample of tissue homogenate or extract in life sciences study. In succession to the cloning of mouse GAPDH gene in the previous course of molecular biology experiment,the transformed E. coli stain was induced by IPTG and the cell lysate was used as a start material for the experimental teaching. The expression of cloned gene was analyzed by rapid dot blot with a specific antibody. Through the class,the student can learn the technique of western blot,observed the final outcome of their molecular cloning experiment directly,their enthusiasm in learning was stimulated and their scientific researching ability were promoted.

关 键 词：分子生物学实验教学 原核表达 斑点印渍 

分 类 号：G642.0[文化科学—高等教育学]