赖氨大黄酸对胆汁淤积性肝纤维化大鼠Smad2、Smad3表达的影响  

Effects of rhein lysine on expression of Smad2 and Smad3 in the model of cholestatic rat with liver fibrosis

作　　者：王琳[1] 郝晓方 张荣花[1] 王密斯 孔艺璇[1] 冯雪妍 章广玲[1,3] 

机构地区：[1]华北理工大学基础医学院病原生物学与免疫学教研室,河北唐山063000 [2]华北理工大学附属医院检验科,河北唐山063000 [3]河北省唐山市慢性病临床基础研究重点实验室,063000

出　　处：《检验医学与临床》2016年第16期2233-2235,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81201281);河北省自然科学基金资助项目(H2013209180;C2012401037;H2013209040);河北唐山市科技计划资助项目(12140209A-4);华北理工大学研究生创新项目(2015S16;2015S17);国家级;省级大学生创新项目(201410081021)

摘　　要：目的探讨赖氨大黄酸(RHL)对胆汁淤积性肝纤维化模型大鼠Smad2、Smad3表达的影响。方法采用胆总管结扎(BDL)的手术方法建立大鼠胆汁淤积性肝纤维化模型,将大鼠随机分为5组:对照组、模型组、赖氨酸组、低剂量RHL组[35mg/(kg·d)]、高剂量RHL组[70mg/(kg·d)]。应用实时荧光定量PCR的方法检测大鼠肝脏组织中Smad2、Smad3mRNA的相对表达量。Western blot的方法检测Smad2、Smad3蛋白时相对表达量的变化。结果与模型组大鼠比较,经RHL治疗后胆汁淤积性肝纤维化大鼠肝脏组织中Smad2、Smad3mRNA相对表达量降低,差异有统计学意义(P<0.05);与模型组大鼠相比,经RHL治疗后胆汁淤积性肝纤维化大鼠肝脏组织中Smad2、Smad3蛋白的相对表达量降低,差异有统计学意义(P<0.05)。结论 RHL能够抑制肝纤维化的发展,可能是通过抑制Smad2、Smad3mRNA和蛋白的表达,阻断TGF-β1/Smads信号传导通路实现的。Objective To observe the effects of rhein lysine on expression of Smad2 and Smad3 in the model of cholestatic rat with liver fibrosis .Methods The model of cholestatic hepatic fibrosis rats was established by ligation of bile duct .The rats were as-signed to the following five groups ：control group ,model group ,lysine group ,lower RHL dose group[35 mg/（kg ± d）] and higher RHL dose group[70 mg /（kg ± d）] .The expression of Smad2 and Smad3 were detected by RT-qPCR and the changes of Smad2 and Smad3 were detected by Western blot .Results RT-qPCR showed that compared with the model group ,the expressions of mRNA of Smad2 and Smad3 in both lower RHL dose group and higher RHL dose group were significantly higher （P〈 0 .05） .Western blot showed that compared with model group the expressions in both lower RHL dose group and higher RHL dose group were also sig-nificantly higher （P〈 0 .05） .Conclusion RHL can inhibit the activation and proliferation of cholestatic liver fibrosis .And this re-sult may due to the inhibitory effect on conduction of TGF-β1/Smadss signal as well as the protein and mRNA of Smad2 and Smad3 .

关 键 词：胆汁淤积 肝纤维化 赖氨大黄酸 SMAD2 SMAD3 

分 类 号：R285.5[医药卫生—中药学]