γ干扰素对变应性鼻炎大鼠鼻腔灌洗液中嗜酸粒细胞凋亡及Eotaxin水平的影响  

Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats

作　　者：李钦[1] 陈彦林[1] 马焱燚[1] 张永东[2] 

机构地区：[1]临沂市人民医院耳鼻咽喉科,山东临沂276003 [2]山东医学高等专科学校药理学教研室,山东临沂276002

出　　处：《山东大学耳鼻喉眼学报》2016年第4期30-33,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：山东省自然科学基金资助项目(ZR2013HL020)

摘　　要：目的建立大鼠变应性鼻炎（allergic rhinitis,AR）模型,探讨鼻腔滴入γ干扰素（interferon gamma,IFN-γ）对AR大鼠鼻腔灌洗液中嗜酸粒细胞（eosinophil,EOS）凋亡及嗜酸粒细胞趋化因子（Eotaxin）水平的影响。方法将40只Wistar大鼠按随机数字表法分为对照组（A组）、AR组（B组）、布地奈德组（C组）和IFN-γ组（D组）,每组10只。B、C和D组大鼠采用卵清蛋白（OVA）、氢氧化铝建立AR大鼠模型,分别于模型建立后第31-38天,每只每日每侧鼻腔滴入磷酸盐缓冲液50μL、布地奈德50μL（1.28μg/μL）,IFN-γ50μL（0.02μg/μL）。A组以生理盐水代替OVA。各组大鼠于第39天取鼻腔灌洗液,检测其EOS百分比,流式细胞术检测EOS凋亡率,酶联免疫吸附试验（ELISA）检测Eotaxin水平。结果 C、D组大鼠鼻腔灌洗液中EOS百分比明显低于B组（P均〈0.01）;C、D组鼻腔灌洗液中EOS凋亡率明显高于B组（P均〈0.01）;C、D组鼻腔灌洗液中Eotaxins明显低于B组。D组大鼠鼻腔灌洗液中EOS百分比与其凋亡率呈显著负相关（r=-0.769,P〈0.05）;D组大鼠鼻腔灌洗液中EOS百分比与Eotaxin水平呈显著正相关（r=0.750,P〈0.05）。结论 IFN-γ可显著增加AR大鼠鼻腔灌洗液中EOS的凋亡,降低Eotaxins水平,明显减少了鼻腔内EOS的浸润,从而减轻了AR的炎症反应,对AR有一定的治疗作用。Objective To explore the effect of interferon gamma（ IFN-γ） on the apoptosis of Eosinophils and Eotaxin levels from nasal lavage fluid in allergic rhinitis（ AR） rats. Methods AR model was established by the sensitizer of ovalbumin and aluminum hydroxide. The 40 rats were randomly divided into the control group（ group A,n = 10）,the allergic rhinitis group（ group B,n = 10）,the budesonide group（ group C,n = 10） and the IFN-γ group（ group D,n =10）. In days 31 to 38 after the establishment of AR rats,each rat of the group D was administrated by instillation of 1 μg（ 1 μg /50 μL） IFN-γ into each of the nasal cavities one time a day,while each rat of the group B was administrated by instillation of 50 μL phosphate buffer in the same way,and each rat of the group C was administrated by instillation of50 μL（ 1. 28 μg / μL） budesonide in the same way. In days 39,the nasal lavage fluid was obtained to measure the apoptotic percentage of eosinophils and the apoptotic ratios were analyzed by flow cytometry. The levels of Eotaxin were detected by enzyme-linked immunosorbent assay（ ELISA）. Results The percentage of EOS in nasal lavage fluid of group C and group D was evidently lower than that of group B（ all P〈0. 01）. The eosinophil apoptotic ratio in group C and group D was evidently higher than that in group B（ all P〈0. 01）. The levels of Eotaxin in nasal lavage fluid of group C and group D were evidently lower than those in group B（ all P〈0. 01）; Furthermore significantly negative correlation was observed betw een the percentage of EOS and apoptotic ratio in the nasal lavage fluid of group D（ r =- 0. 769,P〈0. 05）. While significantly positive correlation was observed betw een the percentage of EOS and Eotaxin in the nasal lavage fluid of group D（ r = 0. 750,P〈0. 05）. Conclusion IFN-γcan significantly reduce the eosinophil infiltration and increase eosinophil apoptosis. It can reduce the levels of Eotaxin so as to achieve the purpose of treating alle

关 键 词：模型 变应性鼻炎 嗜酸粒细胞 凋亡 嗜酸粒细胞趋化因子 鼻腔灌洗液 Γ干扰素 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]