1,4-二氢吡啶类化合物对CHO-K1细胞的辐射防护作用  

Radioprotective effect of 1,4-dihydropyridine in CHO-K1 cell

作　　者：张宇睿[1] 薛虚慧 李园园[1] 徐文清[1] 

机构地区：[1]北京协和医学院&中国医学科学院放射医学研究所天津市放射医学与分子核医学重点实验室,天津300192

出　　处：《辐射研究与辐射工艺学报》2016年第4期40-45,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(81273005);天津市应用基础与前沿技术研究重点项目(14JCZDJC36400);中国医学科学院放射医学研究所发展基金项目(SF1528)资助~~

摘　　要：为探究1,4-二氢吡啶类化合物对于CHO-K1细胞的体外防护活性,实验分为DHP(2,6-二甲基-3,5-二乙酯基-l,4-二氢吡啶)给药照射组(DHP)、单纯照射组(Rad)和对照组(Con)。采用中性红法测定DHP对CHO-K1细胞的细胞毒性和照射后细胞的存活率;DCFH-DA法检测活性氧(Radical oxidative spices,ROS)水平;彗星实验测定DNA损伤情况。通过以上指标来评价DHP的辐射防护活性。结果显示,与Con相比,Rad的ROS水平、DNA百分含量和尾距等指标均上升,并且ROS探针荧光强度达到1 800,尾部DNA含量和尾距分别达到3.5%和1.7%,其差异具有统计学意义(p<0.05),提示照射后CHO-K1细胞中产生了过多的ROS和DNA链断裂损伤。与Rad相比,DHP的ROS探针荧光强度降为945、尾部DNA含量和尾距分别降为0.83%和0.7%,3个指标均明显降低,其差异均具有统计学意义(p<0.05),提示DHP给药能显著降低照射引起的CHO-K1细胞ROS水平升高,通过清除照射产生的过多ROS,减轻照射所致的DNA损伤,发挥细胞保护作用。这说明DHP具有良好的辐射防护作用。The aim is to study the radiation protection effect of 1,4-dihydropyridine in CHO-KI cell. There were three groups which were DHP treated group, radiation group and control group, respectively. Diethyl 2,6- dimethyl-l,4-dihydropyridine-3,5-dicarboxylate （DHP） cell viability and cell cytotoxicity were measured by neutral red assay, DCFH-DA was used to test the level of reactive oxygen species （ROS） and comet assay was adopted to measure DNA damages. Radioprotective activity was evaluated by these three indices. The fluorescence intensity of DCF was 1 800, tail DNA and tail moment were 3.5% and 1.7% in radiation group, which significantly increased （p〈0.05） compared with control group. The results indicated that radiation could produce more ROS to cause DNA damage. The fluorescence intensity of DCF was 945, tail DNA and tail moment were 0.83% and 0.7% in DHP treated group, which significantly decreased （p〈0.05） compared with radiation group. The results indicated that radiation could induce DNA damage and DHP could relief this injury to DNA. It can be concluded that DHP could protect cell against radiation-induced damage in CHO-K1 cell.

关 键 词：1 4-二氢吡啶类化合物 辐射防护剂 活性氧自由基 CHO—K1细胞 

分 类 号：R96[医药卫生—药理学] TL7[医药卫生—药学]