HPLC法同时测定紫锥菊维生素C胶囊中菊苣酸和维生素C的含量  

Simultaneous Determination of Cichoric Acid and Vitamin C in Echinacea and Vitamin C Capsules by HPLC

作　　者：韩凌飞[1] 侯德胜[1] 柳文媛[1] 

机构地区：[1]中国药科大学药物分析教研室,南京210009

出　　处：《海峡药学》2016年第8期58-60,共3页Strait Pharmaceutical Journal

基　　金：国家级大学生创新创业训练计划项目"大学生创新药物研制能力提高项目National Found for Fostering Talents of Basic Science(NFFTBS)"(J1030830)

摘　　要：目的 建立同时测定紫锥菊维生素C胶囊中菊苣酸和维生素C含量的HPLC方法。方法 采用L9（34）正交试验优化提取条件。采用Hedera ODS-2 C18色谱柱（4.6mm×250mm,5μm）,柱温35℃,流动相甲醇-乙腈（1∶1）-0.1%磷酸梯度洗脱,流速1.0m L·min-1,检测波长246nm。结果 维生素C和菊苣酸质量浓度分别在5~100μg·m L-1（r=0.9996）和10~200μg·m L-1（r=0.9996）范围内呈良好线性关系,平均加样回收率（n=9）分别为98.60%（RSD=2.53%）和102.08%（RSD=2.14%）。结论 本方法操作简便,准确度、精密度、稳定性良好,可用于紫锥菊维生素C胶囊的质量控制。OBJECTIVE To establish a method for simultaneous determination of cichoric acid and vitamin C in Echinacea and vitamin C capsules by HPLC. METHODS The L9 （3^4） orthogonal design was carried out in order to optimize the extraction method. The HPLC analysis was performed on a Hedera ODS-2 C18 column（4.6mm× 250mm, 5μm） at 35℃ ,with 0. 1% phosphoric acid and methanol-acetonitrile （1：1 ） as the mobile phase by gradient elution at a flow rate of 1.0mL · min-1. The detection wavelength was 246nm. RESULTS The good linearity of vitamin C and cichoric acid was achieved in the range of 5 - 100μg · mL-1 （ r = 0. 9996 ） and 10 - 200μg· mL-1 （ r = 0. 9996） ;the average recoveries （n =9） were 98.60% （RSD =2.53% ） and 102.08% （RSD =2. 14% ）respectively. CONCLUSION The method is simple and proved to be accurate, reproducible and stable. It could be used to control the quality of Echinacea and vitamin C capsules.

关 键 词：紫锥菊维生素C胶囊 维生素C 菊苣酸 正交试验 同时测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]