黄芪多糖对糖尿病心肌病大鼠的治疗作用及机制探讨  

Protective effect of Astragalus polysaccharides on myocardial inflammation of rat models with diabetic cardiomyopathy

作　　者：陈红霞[1] 曹霞[1] 卢红[1] 汪蕾[2] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100 [2]湖北中医药大学检验学院

出　　处：《山东医药》2016年第30期17-19,共3页Shandong Medical Journal

基　　金：湖北省教育厅科学研究计划资助项目(B2015085)

摘　　要：目的探讨黄芪多糖(APS)治疗糖尿病心肌病(DCM)的机制。方法选择Wistar大鼠50只,其中30只腹腔注射链脲佐菌素制备糖尿病模型后,选择其中20只随机分为模型组、治疗组各10只;其余20只未造模大鼠随机分为空白对照组、APS组各10只。治疗组及APS组予腹腔注射APS 300 mg/(kg·d),空白对照组及模型组予腹腔注射等量生理盐水。分别于第8、10、12、14周末取各组尾静脉血检测空腹血糖。于第14周末抽取腹主动脉血,采用酶联免疫分析法检测血清IL-6、血管紧张素Ⅱ(AngⅡ)水平。取左心室肌组织,采用TUNEL法观察心肌细胞凋亡情况,计算心肌细胞凋亡指数(AI)。结果模型组各时点血糖水平均高于空白对照组(P均<0.05),治疗组第12、14周末血糖水平低于模型组(P均<0.05)。模型组血清IL-6、AngⅡ水平均高于空白对照组及治疗组(P均<0.05)。空白对照组、APS组、模型组、治疗组AI分别为(1.47±0.34)、(1.38±0.56)、(62.56±11.32)、(31.05±8.75)个,模型组心肌细胞AI均高于空白对照组及治疗组(P均<0.05)。结论 APS可降低糖尿病大鼠血清IL-6、AngⅡ水平,抑制心肌细胞凋亡,可能是其治疗DCM的机制之一。Objective To investigate the mechanism of Astragalus polysaccharide（ APS） in the treatment of diabetic cardiomyopathy（ DCM）. Methods Fifty healthy male Wistar rats were selected,30 of which were intraperitoneally injected with STZ to make the diabetic models,and 20 of them were randomly divided into the model group and the treatment group,each had 10 rats. The other 20 rats were randomly divided into the blank control group and the APS group,10 in each. The treatment group and the APS group were administered with 300 mg /（ kg·d） APS by intraperitoneal injection.The blank control group and model group were administered with the same amount of normal saline by intraperitoneal injection. At the end of week 8,10,12 and 14,we took the venous blood to test fasting blood-glucose. At the end of week 14,the blood from abdominal aorta was extracted,and using enzyme-linked immunoassay to detect the levels of serum IL-6 and Ang Ⅱ. The left ventricular muscle tissues were collected,and using TUNEL method to observe the myocardial apoptosis,and to calculate the myocardial apoptosis index（ AI）. Results The blood glucose levels in the model group at different periods were higher than those of the control group（ all P〈 0. 05）,and the blood glucose level of the treatment group at the end of week 12 and 14 was lower than that of the model group（ all P〈 0. 05）. The serum levels of IL-6 and Ang Ⅱ in the model group were higher than those of the blank control group and treatment group（ all P〈 0. 05）. AI of the blank control group,APS group,model group and treatment group were respectively 1. 47 ± 0. 34,1. 38 ± 0. 56,62. 56 ± 11. 32 and31. 05 ± 8. 75,and the myocardial cell AI in the model group were higher than those of the blank control group and treatment group（ all P〈 0. 05）. Conclusion APS can reduce the serum levels of IL-6 and Ang Ⅱ in diabetes mellitus rat models and inhibiting the myocardial apoptosis may be one of the mechanisms in treatment of DCM.

关 键 词：黄芪多糖 糖尿病心肌病 白细胞介素-6 血管紧张素Ⅱ 细胞凋亡 

分 类 号：R587.2[医药卫生—内分泌] R542.2[医药卫生—内科学]