鸭源鸡杆菌整合子及其与耐药性的相关性分析  

Gallibacterium anatis Integron and Its Correlation with the Drug Resistance

作　　者：彭志锋[1] 高冬生[1] 刘红英[1] 杨霞[1] 赵军[1] 王新卫[1] 李永涛[1] 陈陆[1] 常洪涛[1] 王川庆[1] 

机构地区：[1]河南农业大学禽病研究所,郑州450002

出　　处：《畜牧兽医学报》2016年第8期1676-1681,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31302090);河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)

摘　　要：为了解鸭源鸡杆菌的整合子及其与耐药性之间的关系,运用PCR方法检测鸭源鸡杆菌分离株携带1、2、3类整合子的情况,采用K-B法测定61株鸭源鸡杆菌对13种抗菌药的敏感性,并分析其整合子与耐药性的关联性。结果显示,所有参试菌株对克林霉素、诺氟沙星、链霉素有耐药性的菌株比例均不低于88.52%(54/61)。19株鸭源鸡杆菌携带1类整合子,阳性率为31.15%,且此类菌株的耐药种数均≥3;在耐药种数≥5的菌株中,整合子阳性菌的比例(78.95%)显著高于整合子阴性菌的比例(28.57%,P<0.01)。本试验首次研究了鸭源鸡杆菌中整合子的分布情况。参试菌株仅携带1类整合子,且普遍存在多重耐药性;1类整合子可能在鸭源鸡杆菌多重耐药性的形成中发挥一定作用。The aim of the present study was to confirm existence of the integrons in Gallibacterium anatis（G.anatis）isolates and the role of integrons in multidrug resistance.The integrons class1,2and 3in G.anatisisolates were detected by PCR amplification for the first time.The sensitivity of 61 G.anatis strains was tested against 13 kinds of antibiotics by Kirby-Bauer method.The correlation between multidrug resistance and integrons was analyzed.The results showed that drug resistance rates of all G.anatis isolates to clindamycin,norfloxacin and streptomycin were above 88.52%.No less than three kinds of drugs were resisted for 19 G.anatis strains which carrying class 1integron.Among strains resisting more than five kinds of drugs,the proportion of the class 1integron positive（78.95%）was significantly higher than that（28.57%）of class 1integron negative strains（P〈0.01）.It was the first time,to our knowledge,that the distribution of intergons in G.anatis had been investigated.Only class 1integron was found and multidrug resistance was very common in G.anatisisolates in this study.Class 1integron might play an important role in the formation of multiple drug resistance phenotype of G.anatis strains.

关 键 词：鸭源鸡杆菌 整合子 耐药性 

分 类 号：S852.612[农业科学—基础兽医学]