牛源犬新孢子虫对BABL/c孕鼠胎盘激素和细胞因子调节功能的影响  

Imbalance of Placental Hormones and Cytokines Regulation in Pregnant BALB/c Mice Infected by Bovine Neospora caninum

作　　者：贾立军[1] 马莉[1,2] 张守发[1] 李男礼[1] 赵鹏[1] 李积旭 

机构地区：[1]延边大学农学院动物医学系,延吉133000 [2]辽宁省凌海市动物疫病预防控制中心,凌海121200

出　　处：《畜牧兽医学报》2016年第8期1689-1695,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31160501;31360605);吉林省重点科技攻关项目(20140204078NY);吉林省教育厅重点项目(吉教科合字[2015]第1号);吉林省自然科学基金(20160101201JC)

摘　　要：旨在探讨牛源犬新孢子虫对孕鼠胎盘激素和细胞因子调节功能的影响。以牛源犬新孢子虫BABL/c孕鼠感染模型为研究对象,同时设置正常对照组孕鼠,在攻虫第12、14、16、18天分批处死孕鼠,采集孕鼠血清,制备孕鼠胎盘细胞悬液,采用ELISA方法对牛源犬新孢子虫感染孕鼠血清中CRH、CG、PRL及E3等胎盘激素水平,以及孕鼠胎盘细胞悬液中IFN-γ、IL-4及TGF-β等细胞因子水平进行检测。结果显示,与正常对照组孕鼠比较,牛源犬新孢子虫感染孕鼠模型随攻虫时间的增加,在孕鼠血清中,CRH水平在第12天时显著升高(P<0.05),在第18天时显著下降(P<0.01);CG水平在第12天时显著下降(P<0.01),在第16天时显著升高(P<0.05);PRL水平在第18天时显著下降(P<0.05);E3水平在第12天时显著升高(P<0.05),第16天时显著下降(P<0.05)。在孕鼠胎盘细胞悬液中,IFN-γ水平在第18天时显著升高(P<0.05);IL-4水平在第12天时显著升高(P<0.05);TGF-β水平在第12天时显著升高(P<0.05)。结果表明牛源犬新孢子虫感染导致孕鼠胎盘激素和细胞因子的调节功能失衡,严重影响胎盘血流、胎盘物质转运及胎儿生长发育,将导致不良妊娠的发生。To invest the affection on regulating function of pregnant BALB/c mice placental hormones and cytokines caused by bovine Neospora caninum,in present study,we established the pregnant BALB/c mice model infected with bovine Neospora caninum,and pregnant BALB/c mice non-infected as negative control group.For collecting serum,mice of each group were sacrificed on the 12 th,14th,16 th,18th day after infection.The placental hormones CRH,CG,PRL and E3 in serum,and cytokines IFN-γ,IL-4and TGF-βin placental cell suspension from pregnant mice were detected using ELISA method.Compared with control group,the results showed that CRH levels in pregnant BALB/c mice serum was significantly increased（P〈0.05）on the 12 th day and decreased significantly（P〈0.01）on the 18 th day after infection;while GC levels was decreased significantly（P〈0.01）on the 12 th day and increased significantly（P〈0.05）on the 16 th day;PRL levels was decreased significantly（P〈0.05）on the 18 th day;E3levels was significantly increased（P〈0.05）on the 12 th day and decreased significantly（P〈0.05）on the 16 th day.At the sametime,IFN-γlevels in pregnant BALB/c mice placental cell suspension was increased significantly（P〈0.05）on the 18 th day;while IL-4and TGF-βlevels were both increased significantly（P〈0.05）on the 12 th day.These results indicated that infection by bovine Neospora caninumin pregnant mice would lead to imbalance of placental hormones and cytokines regulative function and seriously affect placental blood flow,placenta mass transport,fetal growth and development.All these will lead to adverse pregnancy outcomes.

关 键 词：犬新孢子虫 BABL/c孕鼠 胎盘 激素 细胞因子 

分 类 号：S852.723[农业科学—基础兽医学]