白蛾周氏啮小蜂毒液对美国白蛾蛹血细胞免疫的影响  

Effects of venom of Chouioia cunea( Hymenoptera: Eulophidae) on the hemocyte immunity of Hyphantria cunea( Lepidoptera: Arctiidae) pupae

作　　者：辛蓓[1] 张少斌[2] 刘佩旋[1] 郑雅楠[1] 

机构地区：[1]沈阳农业大学林学院,沈阳110866 [2]沈阳农业大学生物技术学院,沈阳110866

出　　处：《昆虫学报》2016年第7期699-706,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金青年基金项目(31200489)

摘　　要：【目的】明确白蛾周氏啮小蜂Chouioia cunea毒液对其寄主美国白蛾Hyphantria cunea蛹细胞免疫的影响。【方法】采用Na2-EDTA分离美国白蛾蛹颗粒细胞,尼龙毛法分离浆血细胞,再利用细胞离体培养法,测评了白蛾周氏啮小蜂毒液对寄主美国白蛾两种血细胞包囊作用和吞噬作用的影响。【结果】美国白蛾颗粒细胞的包囊指数强于浆血细胞。白蛾周氏啮小蜂毒液对颗粒细胞和浆血细胞的包囊指数均有明显的抑制作用,毒液浓度越大,抑制作用越强,两种血细胞的包囊作用均呈先增长后降低的趋势。在所有浓度毒液处理下,颗粒细胞的包囊指数在12 h时最强。未经小蜂毒液处理的浆血细胞包囊指数在15 h时达到最强,但经浓度为0.01-0.03 VRE/μL的毒液处理后浆血细胞的包囊指数在12 h时达到最强,而经浓度为0.04-0.10 VRE/μL的毒液处理后包囊指数在9 h时最强。白蛾周氏啮小蜂毒液对美国白蛾蛹颗粒细胞的吞噬作用强于浆血细胞。毒液对两种血细胞的吞噬能力均有明显的抑制作用,但毒液处理对浆血细胞的吞噬作用影响较小。【结论】白蛾周氏啮小蜂毒液可以抑制美国白蛾蛹颗粒细胞和浆血细胞的包囊作用和吞噬作用,且随着毒液浓度的增加,两种血细胞的免疫作用显著下降。【Aim】This study aims to clarify the effects of Chouioia cunea venom on the cellular defense of hemocytes in Hyphantria cunea pupae. 【Methods】Granulocytes of H. cunea pupa were separated by Na2-EDTA treatment,and plasmatocytes were separated by nylon wool method. Cell culture in vitro was used to assess the effects of parasitoid C. cunea venom on encapsulation and phagocytosis of host H.cunea hemocytes. 【Results】The encapsulation capacity of granulocytes of H. cunea was stronger than that of plasmatocytes. The encapsulation capacity of hemocytes was significantly inhibited by the parasitoid venom,and deceased gradually with the increase of venom concentration. The encapsulation capacity of both plasmatocytes and granulocytes increased at the beginning and then decreased gradually during the treatment of parasitoid venom. The encapsulation capacity of granulocytes in the treatment groups with different concentrations of venom reached to the maximum at 12 h after treatment. In the control group,the encapsulation capacity of plasmatocytes reached to the strongest at 15 h. However,the encapsulation capacity of plasmatocytes was the strongest at 12 h when they were treated by 0. 01- 0. 03 VRE / μL parasitoid venom,while it was the strongest at 9 h when they were treated by 0. 04- 0. 10 VRE / μL venom. The phagocytosis of C. cunea venom on granulocytes of H. cunea pupae was stronger than that on plasmatocytes. The phagocytosis capacity of hemocytes was significantly inhibited by the parasitoid venom. However,the inhibition of plasmatocytes by the parasitoid venom was not as strong as that of granulocytes. 【Conclusion】The venom of C. cunea can inhibit the encapsulation and phagocytosis of the granulocytes and plasmatocytes of H. cunea,and the cellular immunity significantly decreases with the increase of venom concentration.

关 键 词：白蛾周氏啮小蜂 美国白蛾 毒液 细胞免疫 血细胞 包囊作用 吞噬作用 

分 类 号：Q966[生物学—昆虫学]