西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响  

Effects of kidney bean damaged by Frankliniella occidentalis( Thysanoptera:Thripidae) or Tetranychus urticae( Acari: Tetranychidae) on the activities of protective and detoxification enzymes in the other subsequent herbivore of both

作　　者：郅军锐[1] 田甜[1,2,3] 温娟[1] 刘勇[1] 

机构地区：[1]贵州大学昆虫研究所,贵州省山地农业病虫害重点实验室,贵阳550025 [2]南京农业大学植物保护学院,南京210095 [3]江苏省农业科学院植物保护研究所,南京210014

出　　处：《昆虫学报》2016年第7期707-715,共9页Acta Entomologica Sinica

基　　金：贵州省普通高等学校系统与应用蜱螨学创新团队(黔教合人才团队字([2014]33);贵州省农业攻关项目(黔科合NY[2015]3014-1号)

摘　　要：【目的】害虫取食后会导致植物的防御反应;取食同种植物的不同种害虫其生理适应性可能不同。本研究旨在阐明一种害虫取食后的植物对后取食的另一种害虫虫体酶活性的影响。【方法】采用生化分析法研究了西花蓟马Frankliniella occidentalis 2龄若虫或二斑叶螨Tetranychus urticae雌成螨为害后的菜豆对后取食不同时间下的二斑叶螨第2若螨和雌成螨及西花蓟马2龄若虫和雌成虫体内保护酶[过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)]和解毒酶[多功能氧化酶(MFO)、羧酸酯酶(Car E)、谷胱甘肽-S-转移酶(GSTs)和乙酰胆碱酯酶(Ach E)]活性的影响。【结果】二斑叶螨雌成螨取食西花蓟马为害菜豆5 h时,POD,CAT和SOD这3种保护酶的活性均明显高于取食健康植株(P<0.01),取食蓟马为害的菜豆18 h时只有POD活性低于取食健康植株(P<0.01);二斑叶螨第2若螨取食蓟马为害菜豆后只有CAT活性在5 h时明显高于取食健康植株(P<0.01)。二斑叶螨雌成螨取食蓟马为害菜豆5 h时,解毒酶MFO和Car E的活性受到抑制(P<0.01),但GSTs和Ach E活性均比取食健康菜豆高(P<0.01);取食18 h时,只有MFO的活性变化与5 h不同。第2若螨取食蓟马为害菜豆只有MFO的活性不论在5 h还是18 h时明显高于取食健康菜豆(P<0.01)。西花蓟马取食二斑叶螨雌成螨为害菜豆时,雌成虫体内的3种保护酶的活性不论取食5 h还是18 h均明显升高(P<0.01),但2龄若虫只有CAT活性在这两个时间段下明显升高(P<0.01)。西花蓟马雌成虫取食螨害菜豆5 h时,解毒酶活性的变化同二斑叶螨雌成螨,取食18 h时只有GSTs活性变化状态与取食5 h不同;西花蓟马2龄若虫取食螨害菜豆时除GSTs外解毒酶的活性变化在这两个时间下正好相反。【结论】西花蓟马或二斑叶螨为害的菜豆能诱导后取食的二斑叶螨和西花蓟马产生一系列应激生化反应,且两种害虫成虫体内所有�【Aim】Insect feeding can induce the defense responses of host plants. Different species feeding on the same host plant may have different physiological adaptation. This study aims to investigate the effects of plant damaged by one pest on the enzyme activities in the other subsequent herbivore.【Methods】Biochemistry analysis method was used to study the changes in activities of protective enzymes[peroxidase（ POD）,catalase（ CAT）,and superoxide dismutase（ SOD） ] and detoxification enzymes[mixed-function oxidase（ MFO）,carboxylesterase（ Car E）,glutathione S-transferase（ GSTs） and acetylcholin esterase（ Ach E） ] in deutonymphs and female adults of Tetranychus urticae at different time points after feeding on kidney bean plants damaged by the 2nd instar nymphs of Frankliniella occidentalis and in the 2nd instar nymphs and female adults of F. occidentalis at different time points after feeding on bean plants damaged by female adults of T. urticae. 【Results】The activities of POD,CAT and SOD in female adults of T. urticae at 5 h after feeding on bean plants damaged by F. occidentalis（ BPDF） were significantly higher than those after feeding on healthy bean plants（ HBP）（ P〈0. 01）,whereas the POD activity in female adults at 18 h after feeding on BPDF was lower than that after feeding on HBP（ P〈0. 01）. The CAT activity in deutonymphs of T. urticae at 5 h after feeding on BPDF was significantly higher than that after feeding on HBP（ P〈0. 01）. At 5 h after feeding on BPDF,the activities of MFO and Car E in female adults of T. urticae were suppressed（ P〈0. 01）,while the GSTs and Ach E activities were higher than those after feeding on HBP（ P〈0. 01）. At 18 h after feeding on BPDF,only MFO activity was different from that at 5 h. Only MFO activities in deutonymphs of T.urticae at 5 h or 18 h after feeding on BPDF were higher than those after feeding on HBP（ P〈0. 01）.The activities of POD,CAT,and SOD in female adults of F. occidentalis at 5 h or 18

关 键 词：二斑叶螨 西花蓟马 菜豆 保护酶 解毒酶 酶活性 生理适应性 

分 类 号：Q965[生物学—昆虫学]