糖化血红蛋白对血红蛋白H病患者的适用性研究  

The applicability of glycated hemoglobin A_(1c) for patients with hemoglobin H disease

作　　者：徐安平[1] 纪玲[1] 陈卫东[1] 夏勇[1] 喻晶[1] 周宇[1] 李璐[1] 

机构地区：[1]北京大学深圳医院检验科,广东深圳518036

出　　处：《检验医学》2016年第8期667-670,共4页Laboratory Medicine

基　　金：深圳市科技计划创新项目(JCYJ20140415162338838)

摘　　要：目的探讨血红蛋白H(HbH)病对4种糖化血红蛋白(HbA_(1c))检测系统的影响,以及HbA_(1c)检测系统是否适用于HbH病患者。方法收集HbH病样本27例,对照组样本30例。采用亲和层析高压液相色谱法、毛细管电泳法、免疫透射比浊法和离子交换高压液相色谱法进行HbA_(1c)检测,以亲和层析高压液相色谱法为参比系统,比较不同检测系统HbA_(1c)结果的差异。同时检测空腹血糖(FG)和糖化血清蛋白(GSP),比较对照组和HbH样本的FG、GSP、HbA_(1c)和平均血糖的结果差异。结果 3种检测系统与参比系统(Ultra2)的HbA_(1c)结果比较,P值分别为0.08、0.89和0.64,差异均无统计学意义(P>0.05);对照组和HbH样本的FG和GSP结果差异无统计学意义(P>0.05),对照组和HbH样本的HbA_(1c)和平均血糖结果差异有统计学意义(P<0.01)。结论 HbH病患者的HbA_(1c)结果不能代表平均血糖水平,HbA_(1c)指标不适用于此类患者的长期血糖水平监控。Objective To investigate the influence of hemoglobin H （HbH） disease on 4 kinds of glycated hemoglobin A1c（HbA1c） systems and the applicability of HbA1c for patients with HbH disease. Methods A total of 27 samples with HbH disease and 30 control samples were collected. HbA1c determinations were performed by boronate affinity high performance liquid chromatography,capillary electrophoresis,turbidimetric immunoassay and ion exchange high performance liquid chromatography. The difference of the 4 systems was compared. Fasting glucose（FG） and glycated serum protein（GSP） were determined as well. The differences of FG,GSP,HbA1c and average glucose were compared between control and HbH disease groups. Results Compared with boronate affinity high performance liquid chromatography（Ultra2）,P values were 0.08,0.89 and 0.64,with no statistical significance（P〉0.05）. The differences of FG and GSP had no statistical significance between control and HbH disease groups（P〉0.05）. The differences of HbA1c and average glucose had statistical significance between control and HbH disease groups（P〈0.01）. Conclusions HbA1c of HbH disease group can not reflect the real level of average glucose,and it is not applicable for long-term blood glucose monitoring in patients with HbH disease.

关 键 词：糖化血红蛋白 红细胞生命周期 平均血糖 

分 类 号：R556.7[医药卫生—血液循环系统疾病]