江苏盐城地区异育银鲫大红鳃疾病病原学研究及病理学观察  

ETIOLOGY INVESTIGATION AND HISTOPATHOLOGICAL STUDY ON “RED-GILL” OF SILVER PRUSSIAN CARP(CARASSIUS AURATUS GIBELIO) IN YANCHENG CITY

作　　者：刘韬[1] 汪开毓[1,2] 陈俊杰[1] 刘天强 李茂 康岳华 段靖[1] 贺扬[1] 

机构地区：[1]四川农业大学鱼病研究中心,成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,成都611130 [3]通威股份动物保健研究所,成都610000

出　　处：《水生生物学报》2016年第5期928-934,共7页Acta Hydrobiologica Sinica

基　　金：教育部《长江学者和创新团队发展计划》创新团队项目(IRT0848)资助~~

摘　　要：为鉴定近期江苏盐城地区养殖异育银鲫"大红鳃"疾病病原,研究从患病濒死鱼中分离到两株致病菌并对其理化特性、分子生物学、感染病理学进行了研究,同时开展了鉴别诊断和回归试验以及药敏检测。结果显示,两分离株理化特性与温和气单胞菌(A.sobria)特征相符,与Gen Bank中A.sobria的gyr B基因同源性达99%以上,在系统发育树上均与A.sobria聚为一族。同时与鲤科疱疹病毒2型(Cy HV-2)鉴别诊断结果表明,患病鱼组织中Cy HV-2检测为阴性。组织学上,鳃和肾组织具有典型病理变化。人工感染两株A.sobria后,鱼体出现与自然发病相似的"大红鳃"症状,并能从发病鱼组织中再分离到相同病原菌;而人工感染Cy HV-2组织匀浆后的鲫虽然出现死亡,但无"大红鳃"症状出现。综上,确认此次"大红鳃"疾病的病原为A.sobria。药敏实验表明,分离菌株对诺氟沙星、氧氟沙星、氯霉素三种药物敏感。研究为临床上更好的预防异育银鲫"大红鳃"疾病奠定了基础。Two Aeromonas sobria isolates, isolated from cultured Carassius auratus gibelio during an outbreak of mass mortality in Yancheng city of China, were identified to the species level by phylogenetic analysis based on gyrB gene and biochemical tests. The isolates were identified to be the pathogeny of ＂Red-Gill＂ through investigating for the virulence of bacteria by recurrent tests and histopathology. We also described the macroscopic and microscopic lesions in Carassius auratus gibelio naturally infected with A. sobria. Our findings confirmed that the 2 isolates, rather than cyprinid herpesvirus 2, were the primary pathogen of ＂Red-Gill＂ disease and damaged the immune system in Carassius auratus gibelio. The results of drug sensitivity tests showed that the bacterial isolates was sensitive to ofloxacin, chloromycetin and norfloxacin; While, resistant to oxacillin, vancomycin, ampicillin, penicillin, clindamycin etc. Together, our findings demonstrate the pathogenesis of Aeromonas sobria in Carassius auratus gibelio and establish the foundation for ＂Red-Gill＂ in cultured Carassius auratus gibelio.

关 键 词：异育银鲫 温和气单胞菌 大红鳃 CyHV-2 病理组织学 

分 类 号：S941.41[农业科学—水产养殖]